Chapter 6, Problem 26P

Five $\text{rII}$ partial-deletion mutants are mapped and then tested for their ability to form wild $\text{-}$ type recombinants with six point mutants. The extent and endpoints of deletion mutants are shown below the $\text{rII}$ region of the chromosome.

a. Use the data in Table A to place each point mutation as precisely as you can along the chromosome.

b. Use the complementation data in Table B to determine where the division between rIIA and rIIB is located on the rII region.

c. Based on the data and on your analysis, draw a complementation table for the five point mutants $\text{55, 67, 74, 82,}$ and $\text{85}$. (Skip mutant $\text{91}$ for this problem.)

d. Add mutant $\text{91}$ to your complementation table (assume it maps to rIIA).