GENETIC ANALYSIS: AN INTEG. APP. W/MAS
2nd Edition
ISBN: 9781323142790
Author: Sanders
Publisher: Pearson Custom Publishing
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Chapter 6, Problem 16P
Suppose you have an
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When human hemoglobin undergoes a mutation, the
mutant protein usually does not replace all of the normal HbA in
the red blood cells or erythrocytes of the individual. The erythro-
cytes contain mixtures of varying amounts of both HbA and the
mutant protein depending on the mutation and the individual. Hb
Yakima is a mutant human Hb with an Asp-(B99)His mutation.
The diagram on the right shows that Hb Yakima was separated
by DEAE-cellulose chromatography from HbA with a 0 – 0.1 M
linear gradient of NaCl buffered to pH 8.3. Why is chromatog-
raphy carried out at pH 8.3? If the isoelectric point of HbA is 6.85,
what is the change in total charge caused by the mutation?How
does the change in charge explain the chromatography elution
profile of the Hb Yakima/HbA mixture?
1,5
-Hb-A
Hb -Yakima
1.0
0.5-
20
40
60
80
00
Fraction number
O.D578 nm
For each of the E. coli strains containing the lacoperon alleles listed, indicate whether the strain isinducible, constitutive, or unable to expressβ-galactosidase and permease.a. I+ o+ Z− Y+/ I+ ocZ+ Y+b. I+ o+ Z+ Y+/ I− ocZ+ Y−c. I+ o+ Z− Y+/ I− ocZ+ Y−d. I−P− o+ Z+ Y−/ I+ P+ ocZ− Y+e. Iso+ Z+ Y+/ I− o+ Z+ Y−
Genomic DNA from a family where sickle-cell disease is known to be hereditary, is digested with the restriction enzyme MstII and run in a Southern Blot. The blot is hybridised with two different 0.6 kb probes, both probes (indicated in red in the diagram below) are specific for the β-globin gene (indicated as grey arrow on the diagram below). The normal wild-type βA allele contains an MstII restriction site indicated with the asterisk (*) in the diagram below; in the mutated sickle-cell βS allele this restriction site has been lost.
What size bands would you expect to see on the Southern blots using probe 1 and probe 2 for an individual with sickle cell disease (have 2 βS alleles)?
Probe 1
Probe 2
(a)
0.6kb
0.6kb and 1.2kb
(b)
0.6kb and 1.8kb
0.6kb, 1.2kb and 1.8kb
(c)
1.2kb
0.6kb
(d)
1.8kb
1.8kb
a.
(a)
b.
(b)
c.
(c)
d.
(d)
Chapter 6 Solutions
GENETIC ANALYSIS: AN INTEG. APP. W/MAS
Ch. 6 - For bacteria that are F+, Hfr, F', and F-, perform...Ch. 6 - The flow diagram identifies relationships between...Ch. 6 - Conjugation between an Hfr cell and an F-cell does...Ch. 6 - Bacteria transfer genes by conjugation,...Ch. 6 - Explain the importance of the following features...Ch. 6 - Prob. 6PCh. 6 - Describe what is meant by the term site-specific...Ch. 6 - What is a prophage, and how is a prophage formed?Ch. 6 - How is the frequency of cotransduction related to...Ch. 6 - Describe the differences between genetic...
Ch. 6 - Among the mechanisms of gene transfer in bacteria,...Ch. 6 - What is lateral gene transfer? How might it take...Ch. 6 - Lateral gene transfer is thought to have played a...Ch. 6 - Prob. 14PCh. 6 - Prob. 15PCh. 6 - 16. Suppose you have an lysis mutant that maps to...Ch. 6 - Five Hfr strains from the same bacterial species...Ch. 6 - An interrupted mating study is carried out on Hfr...Ch. 6 - An Hfr strain with the genotype cys+leu+met+strS...Ch. 6 - A triple-auxotrophic strain of E. coli having the...Ch. 6 - Penicillin was first used in the 1940 s to treat...Ch. 6 - An attribute of growth behavior of eight...Ch. 6 - Synthesis of the amino acid histidine is a...Ch. 6 - The phage P1 is used as a generalized transducing...Ch. 6 - Prob. 25PCh. 6 - Five rII partial-deletion mutants are mapped and...Ch. 6 - A 2013 CDC report identified the practice of...Ch. 6 - Hfr strains that differ in integrated F factor...
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