Pearson eText Genetic Analysis: An Integrated Approach -- Instant Access (Pearson+)
3rd Edition
ISBN: 9780135564172
Author: Mark Sanders, John Bowman
Publisher: PEARSON+
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Chapter 6, Problem 17P
Five Hfr strains from the same bacterial species are analyzed for their ability to transfer genes to
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By conducting conjugation experiments between Hfr and recipientstrains, Wollman and Jacob mapped the order of many bacterialgenes. Throughout the course of their studies, they identified severaldifferent Hfr strains in which the F-factor DNA had been integratedat different places along the bacterial chromosome. A sample of theirexperimental results is shown in the following table:Analyze data. Compare and contrast. Make a drawing.
Determine the map distances between the genes.
Bacteriophage P22 was used in generalised transduction experiments to infect the Salmonella
typhimurium donor strains described in the table below.
The resulting phage lysates were then used to infect the recipient strains of S. typhimurium
recipient strains listed in the table.
In each cross, a phenotype was selected for one of the selected for one of the three genetic
markers studied (str, aceA, thrA), and were made to select the recombinants corresponding to the
other two markers. markers.
The results are given in the following table:
Strain
I donor
str thrA
aceA thrA
str aceA+
Strain
recipient
strs thrA+
aceA thrA
str aceA
Phenotype
selected
Str
Ace+
Str
recombinants
selected
ThrA
ThrA
ThrA
ThrA
Ace
Ace
Number
60
40
95
5
10
90
str: gene involved in streptomycin resistance,
aceA: gene involved in the use of acetate as a carbon source,
thrA: gene involved in threonine biosynthesis.
1) What are the selective media used in these three transduction experiments? to obtain the
selected…
Chapter 6 Solutions
Pearson eText Genetic Analysis: An Integrated Approach -- Instant Access (Pearson+)
Ch. 6 - For bacteria that are F+, Hfr, F', and F-, perform...Ch. 6 - The flow diagram identifies relationships between...Ch. 6 - Conjugation between an Hfr cell and an F-cell does...Ch. 6 - Bacteria transfer genes by conjugation,...Ch. 6 - Explain the importance of the following features...Ch. 6 - Prob. 6PCh. 6 - Describe what is meant by the term site-specific...Ch. 6 - What is a prophage, and how is a prophage formed?Ch. 6 - How is the frequency of cotransduction related to...Ch. 6 - Describe the differences between genetic...
Ch. 6 - Among the mechanisms of gene transfer in bacteria,...Ch. 6 - What is lateral gene transfer? How might it take...Ch. 6 - Lateral gene transfer is thought to have played a...Ch. 6 - Prob. 14PCh. 6 - A 2013 CDC report identified the practice of...Ch. 6 - Hfr strains that differ in integrated F factor...Ch. 6 - Five Hfr strains from the same bacterial species...Ch. 6 - An interrupted mating study is carried out on Hfr...Ch. 6 - An Hfr strain with the genotype cys+leu+met+strS...Ch. 6 - A triple-auxotrophic strain of E. coli having the...Ch. 6 - Penicillin was first used in the 1940 s to treat...Ch. 6 - An attribute of growth behavior of eight...Ch. 6 - Synthesis of the amino acid histidine is a...Ch. 6 - The phage P1 is used as a generalized transducing...Ch. 6 - Prob. 25PCh. 6 - Prob. 26PCh. 6 - Look closely at the consolidated Hfr map and the...Ch. 6 - Fifty bacterial colonies are on a complete-medium...
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- You discover a new bacteria and are interested in mapping its genome with respect to 10 genes. You are able to infer time-of-entry maps for four Hfr strains, which are pictured below. What is the map of the bacterial genome based on these Hfr experiments? Hfr1 Ga E Ne Hfr2 Sq Lix Tf Ne Hfr3 U I D Ga Hfr4 D U Sq O Sq - Lix - Tf - Ne -E- M- Ga - D -1-U O Sq - Tf - Lix -U -E-D- Ne -D -M- Ga OD-1-U- Sq - Lix - Ga - E -M- Tf - Ne OD- Ne - Ga -E- M - Sq - I-U- Tf - Lix O Lix - Ga - E - Sq -1-U- M -D- Tf - Nearrow_forwardBy conducting conjugation experiments between Hfr and recipientstrains, Wollman and Jacob mapped the order of many bacterialgenes. Throughout the course of their studies, they identified severaldifferent Hfr strains in which the F-factor DNA had been integratedat different places along the bacterial chromosome. A sample of theirexperimental results is shown in the following table:What information do you know based on the question and your understanding of the topic?arrow_forwarda. Bacteriophage P22 was used in generalized transduction experiments to infect the Salmonella typhimurium donor strains described in the table below. The resulting phage lysates were then used to infect the S. typhimurium recipient strains listed in the table. In each cross, a phenotype was selected for one of the three genetic markers studied (str, aceA, thrA), and then replicates were performed to select the corresponding recombinants for the other two markers. The results are given in the following table: Recipient strain Selected phenotype Selected recombinants Donor strain str thrA aceAt thrA str aceA+ strs thrA+ aceA thrA+ str aceA Str Ace+ Str ThrA ThrA+ ThrA ThrA+ Ace Ace+ str: gene involved in streptomycin resistance, aceA: gene involved in the use of acetate as a carbon source, thrA: gene involved in the biosynthesis of threonine. Number 60 40 95 5 10 90 What are the selective media used in these three transduction experiments, on the one hand to obtain the selected…arrow_forward
- E. coli cells are simultaneously infected with two strains of phage λ. One strain has a mutant host range, is temperature sensitive, and produces clear plaques (genotype h st c); another strain carries the wildtype alleles (genotype h+ st+ c+). Progeny phages are collected from the lysed cells and are plated on bacteria. The following numbers of different progeny phages are obtained: Progeny phage genotype Number of plaques h+ c+ st+ 321 h c st 338 h+ c st 26 h c+ st+ 30 h+ c st+ 106 h c+ st 110 h+ c+ st 5 h c st+ 6 a. Determine the order of the three genes on the phage chromosome. b. Determine the map distances between the genes. c. Determine the coefficient of coincidence and the interferencearrow_forwardBacteriophage P22 was used in generalized transduction experiments to infect the Salmonella typhimurium donor strains described in the table below. The resulting phage lysates were then used to infect the S. typhimurium recipient strains listed in the table. In each cross, a phenotype was selected for one of the three genetic markers studied (str, aceA, thrA), and then replicates were performed to select the corresponding recombinants for the other two markers. The results are given in the following table: Recipient strain Selected phenotype Selected recombinants Donor strain str thrA aceA+ thrA str aceA+ strs thrA+ aceA thrA+ str aceA Str Ace+ Str ThrA ThrA+ ThrA ThrA+ Ace Ace str: gene involved in streptomycin resistance, aceA gene involved in the use of acetate as a carbon source, thrA: gene involved in the biosynthesis of threonine. Number 60 40 95 5 10 90 Determine the order of the genes and draw a genetic map showing this orderarrow_forwardWe have two specific strains of E. coli that have shown horizontal gene transfer (HGT) when mixed. To experimentally determine the method of HGT that is happening, the following conditions are set up in different tubes of culture media: A) Donor and recipient strain mixed together (control - no treatment). B) Donor and recipient strains mixed together, DNase added (can digest DNA in solution, not within cells).C) Special tube containing a membrane filter (with pores that allow DNA and viruses to pass through, but not bacterial cells) that separates two compartments. Donor strain is added on one side, the recipient strain on the other (they are separated by the filter).D) Donor and recipient strains mixed together, with chemical that inactivates viruses (chemical affects bacteriophages in solution so they are unable to attach to cells). The results: Tubes A, B, and D: HGT was observed. Tube C: HGT was NOT observed. Based on this, which type of HGT was occurring? Conjugation,…arrow_forward
- Two mutations that affect plaque morphology in phages (a− and b −) have been isolated. Phages carrying both mutations (a− b−) are mixed with wild-type phages (a+ b+) and added to a culture of bacterial cells. Once the phages have infected and lysed the bacteria, samples of the phage lysate are collected and cultured on plated bacteria. The following numbers of plaques are observed: Plaque phenotype Number a+ b+ 2043 a+ b− 320 a− b+ 357 a− b− 2134 What is the frequency of recombination between the a and b genes?arrow_forwardDetermine the order of the three genes on the phage chromosome.arrow_forwardIn Escherichia coli, four different Hfr strains, derived from the same F* strains, were mated with F strains auxotrophic for a number of nutritional requirements (Arg Bio Cys Trp Gal His Lac Mal Xyl Leu Met). Matings were interrupted at various intervals and cells were plated on minimal medium supplemented with particular nutrients to test for gene transfer. The following results show the time of entry for each of the genes in four different Hfr strains. Table 1: Time-of-Entry Mapping Data* Hfr strains Genes lac" his" arg' bio 9. Cys gal" trp' 5 11.5 2.5 mal" xyl" leu met Hfr 1 Hfr 2 Hfr 3 Hfr 4 6.5 3.5 11 15 15 4 6. 17.5 5 14.5 3 20 * The numbers denote the number of minutes elapsed before a gene enters the F cells. Draw a circular map of E. coli chromosome.arrow_forward
- By conducting conjugation experiments between Hfr and recipientstrains, Wollman and Jacob mapped the order of many bacterialgenes. Throughout the course of their studies, they identified severaldifferent Hfr strains in which the F-factor DNA had been integratedat different places along the bacterial chromosome. A sample of theirexperimental results is shown in the following table:Explain how these results are consistent with the idea that thebacterial chromosome is circular?arrow_forwardBy conducting conjugation experiments between Hfr and recipientstrains, Wollman and Jacob mapped the order of many bacterialgenes. Throughout the course of their studies, they identified severaldifferent Hfr strains in which the F-factor DNA had been integratedat different places along the bacterial chromosome. A sample of theirexperimental results is shown in the table:Draw a map that shows the order of genes and the locations ofthe origins of transfer among these different Hfr strains?arrow_forwardDraw a diagram/figure to explain the conjugation process (e.g. use PowerPoint or draw one by hand and include a photo of it). You should include in the diagram the F- recipient, Hfr Donor and the transconjugant/recombinant recipient. Make sure to include the genes encoding for Leucine, Threonine, Thiamine and Streptomycin resistance in your diagram. How does an Hfr strain of coli transfers chromosomal DNA to an F- strain? What determines how much of the chromosomal DNA is transferred?arrow_forward
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