Pearson eText Genetic Analysis: An Integrated Approach -- Instant Access (Pearson+)
3rd Edition
ISBN: 9780135564172
Author: Mark Sanders, John Bowman
Publisher: PEARSON+
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Chapter 6, Problem 27P
Look closely at the consolidated Hfr map and the data used to build the map on page
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Determine the map distances between the genes.
An Hfr strain that is leuA+ and thiL+ was mixed with a strain thatis leuA− and thiL−. In the data points shown in the following graph,the conjugation was interrupted at different time points, and thepercentage of recombinants for each gene was determined bystreaking on a medium that lacked either leucine or thiamine.What is the map distance (in minutes) between these two genes?
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Chapter 6 Solutions
Pearson eText Genetic Analysis: An Integrated Approach -- Instant Access (Pearson+)
Ch. 6 - For bacteria that are F+, Hfr, F', and F-, perform...Ch. 6 - The flow diagram identifies relationships between...Ch. 6 - Conjugation between an Hfr cell and an F-cell does...Ch. 6 - Bacteria transfer genes by conjugation,...Ch. 6 - Explain the importance of the following features...Ch. 6 - Prob. 6PCh. 6 - Describe what is meant by the term site-specific...Ch. 6 - What is a prophage, and how is a prophage formed?Ch. 6 - How is the frequency of cotransduction related to...Ch. 6 - Describe the differences between genetic...
Ch. 6 - Among the mechanisms of gene transfer in bacteria,...Ch. 6 - What is lateral gene transfer? How might it take...Ch. 6 - Lateral gene transfer is thought to have played a...Ch. 6 - Prob. 14PCh. 6 - A 2013 CDC report identified the practice of...Ch. 6 - Hfr strains that differ in integrated F factor...Ch. 6 - Five Hfr strains from the same bacterial species...Ch. 6 - An interrupted mating study is carried out on Hfr...Ch. 6 - An Hfr strain with the genotype cys+leu+met+strS...Ch. 6 - A triple-auxotrophic strain of E. coli having the...Ch. 6 - Penicillin was first used in the 1940 s to treat...Ch. 6 - An attribute of growth behavior of eight...Ch. 6 - Synthesis of the amino acid histidine is a...Ch. 6 - The phage P1 is used as a generalized transducing...Ch. 6 - Prob. 25PCh. 6 - Prob. 26PCh. 6 - Look closely at the consolidated Hfr map and the...Ch. 6 - Fifty bacterial colonies are on a complete-medium...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- This question combines your knowledge of bacterial conjugationand the genetic regulation that directs thephage λ reproductive cycles. When researchers mix donor Hfr strains with recipient F– bacteria that are lysogenic for phage λ, the conjugated cells survive normally. However, if donor Hfr strains that are lysogenic for phage λ conjugate with recipient F– bacteria that do not contain any phage λ, the recipient cells often lyse, due to the induction of λ into the lytic cycle. Based on your knowledge of the regulation of the two reproductive cycles of phage λ, explain this experimental observation.arrow_forwardDraw a diagram/figure to explain the conjugation process (e.g. use PowerPoint or draw one by hand and include a photo of it). You should include in the diagram the F- recipient, Hfr Donor and the transconjugant/recombinant recipient. Make sure to include the genes encoding for Leucine, Threonine, Thiamine and Streptomycin resistance in your diagram. How does an Hfr strain of coli transfers chromosomal DNA to an F- strain? What determines how much of the chromosomal DNA is transferred?arrow_forwardBy conducting conjugation experiments between Hfr and recipientstrains, Wollman and Jacob mapped the order of many bacterialgenes. Throughout the course of their studies, they identified severaldifferent Hfr strains in which the F-factor DNA had been integratedat different places along the bacterial chromosome. A sample of theirexperimental results is shown in the following table:Analyze data. Compare and contrast. Make a drawing.arrow_forward
- Describe the differences between the F factor and Hfr transfer. Include how the donor and recipient are affected in each type of conjugation.arrow_forwardAn Hfr strain that is leuA+ and thiL+ was mixed with a strain thatis leuA− and thiL−. In the data points shown in the following graph,the conjugation was interrupted at different time points, and thepercentage of recombinants for each gene was determined bystreaking on a medium that lacked either leucine or thiamine.What information do you know based on the question and your understanding of the topic?arrow_forwardDraw a diagram/figure to explain the conjugation process (. You should include in the diagram the F- recipient, Hfr Donor and the transconjugant/recombinant recipient. Make sure to include the genes encoding for Leucine, Threonine, Thiamine and Streptomycin resistance in your diagram. How does an Hfr strain of coli transfers chromosomal DNA to an F- strain? What determines how much of the chromosomal DNA is transferred?arrow_forward
- An Hfr strain that is leuA+ and thiL+ was mixed with a strain thatis leuA− and thiL−. In the data points shown in the following graph,the conjugation was interrupted at different time points, and thepercentage of recombinants for each gene was determined bystreaking on a medium that lacked either leucine or thiamine.Make a calculation.arrow_forwardDetermine the order of the three genes on the phage chromosome.arrow_forwardIn Escherichia coli, four different Hfr strains, derived from the same F* strains, were mated with F strains auxotrophic for a number of nutritional requirements (Arg Bio Cys Trp Gal His Lac Mal Xyl Leu Met). Matings were interrupted at various intervals and cells were plated on minimal medium supplemented with particular nutrients to test for gene transfer. The following results show the time of entry for each of the genes in four different Hfr strains. Table 1: Time-of-Entry Mapping Data* Hfr strains Genes lac" his" arg' bio 9. Cys gal" trp' 5 11.5 2.5 mal" xyl" leu met Hfr 1 Hfr 2 Hfr 3 Hfr 4 6.5 3.5 11 15 15 4 6. 17.5 5 14.5 3 20 * The numbers denote the number of minutes elapsed before a gene enters the F cells. Draw a circular map of E. coli chromosome.arrow_forward
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