Biochemistry: Concepts and Connections (2nd Edition)
2nd Edition
ISBN: 9780134641621
Author: Dean R. Appling, Spencer J. Anthony-Cahill, Christopher K. Mathews
Publisher: PEARSON
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Textbook Question
Chapter 6, Problem 15P
It has been postulated that the normal (noninfectious) form of prion differs from the infectious form only in secondary/tertiary structure.
a. How might you show that changes in secondary structure occur?
b. If this model is correct, what are the implications for structural prediction schemes?
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SET 1: PEPTIDE SEQUENCING BY ENZYME DEGRADATION AND EDMANN TECHNIQUE
A. Predict the fragments that will be generated from the treatment of the following peptide with:
a.) trypsin
b.) chymotrypsin
c.) and S. aureus V8 protease
G-A-W-R-N-A-K-E-F-G-Q
WRITE the fragments in this table:
trypsin
chymotrypsin
S. aureus V8 protease
G-A-W-R; N-A-K; E-F-G-Q
G-A-W; R-N-A-K-E-F; G-Q
G-A-W-R-N; A-K-E; F-G-Q
B. The sequence of a 29 aa long peptide can be determined from the following data:
Treatment of the peptide with dansyl chloride reveals that the amino terminal is Val.
а.
b. Trypsin digestion, separation of peptides, and Edmann technique give the sequences for
peptide fragments as follows:
T1 V-G-A-H-A-G-E-Y-G-A-E-A-T-E
C- Terminal Peptide fragment
T2 A-A-W-G-K & T4 T-N-V-K
middle peptide fragments
T3 V-L-S-P-A-K
N-Terminal Peptide fragment
c. Chymotrypsin digestion, separation of peptides, and Edmann technique give the sequences
for peptide fragments as follows:
C1 G-A-E-A-T-E
C- Terminal…
Solve the attached problem
Please give me correct solution for both questions c and d.
Chapter 6 Solutions
Biochemistry: Concepts and Connections (2nd Edition)
Ch. 6 - Prob. 1PCh. 6 - Bovine pancreatic trypsin inhibitor (BPTI; Figure...Ch. 6 - A schematic structure of the subunit of...Ch. 6 - In the protein adenylate kinase, the C-terminal...Ch. 6 - Give two reasons to explain why a proline residue...Ch. 6 - Consider a small protein containing 101 amino acid...Ch. 6 - a. Based on a more conservative answer to Problem...Ch. 6 - The following sequence is part of a globular...Ch. 6 - a. A protein is found to be a tetramer of...Ch. 6 - Under physiological conditions, the protein...
Ch. 6 - Theoretical and experimental measurements show...Ch. 6 - The peptide hormone vasopressin is used in the...Ch. 6 - A protein gives under conditions of buffer...Ch. 6 - A protein gives a single band on SDS get...Ch. 6 - It has been postulated that the normal...Ch. 6 - Below are shown two views of the backbone...Ch. 6 - Do you expect a Pro Gly mutation in a...Ch. 6 - Rank the following in terms of predicted rates...Ch. 6 - Shown below are two cartoon views of the small...Ch. 6 - Prob. 20PCh. 6 - In most cases, mutations in the core of protein...Ch. 6 - A Leu Ala mutation at a site buried the core of...Ch. 6 - Disulfide bonds have been shown to stabilize...Ch. 6 - Cartoon renderings of the proteins Top 7 and adaH2...
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