Biochemistry: Concepts and Connections (2nd Edition)
2nd Edition
ISBN: 9780134641621
Author: Dean R. Appling, Spencer J. Anthony-Cahill, Christopher K. Mathews
Publisher: PEARSON
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Textbook Question
Chapter 6, Problem 23P
Disulfide bonds have been shown to stabilize proteins (i.e., make them less likely to unfold). Consider the cases shown schematically below for two variants of the same protein. In case #1 the disulfide forms between Cys residues that have been introduced near the protein N- and C-termini, and in case #2 the disulfide forms between Cys residues that have been introduced in the middle of the protein sequence. Which protein is likely to be more stable? (Note: Assume the disulfide bond is intact in both the unfolded and folded states). Explain your reasoning.
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You have discovered a novel protein that has a pI = 5.5. To study the functional properties of this new protein, your research group has made a mutant that contains two amino acid changes—namely, a surface Phe residue in the normal protein has been replaced by His (side chain pKa = 6.1) and asurface Gln has been replaced by Glu (side chain pKa = 6.0). Is the pI of themutant protein predicted to be greater than, less than, or the same as the pIof the normal protein? Support your answer with the appropriate calculation
You have discovered a novel protein that has a pl = 5.5. To study the
functional properties of this new protein, your research group has
made a mutant that contains two amino acid changes: a surface
phenylalanine residue in the normal protein has been replaced by
histidine (side chain pK, = 6.1), and a surface glutamine has been
replaced by glutamic acid (side chain pK, = 6.0). Is the pl of the
mutant protein predicted to be greater than, less than, or the same as
the pI of normal protein? Support your answer with the appropriate
calculation.
1) You are studying the toxic protein called ectatomin, a major component of ant venom
(specifically from the species Ectatomma tuberculatum), that embeds into cell
membranes and creates pores that cause cells to lyse. Ectatomin is a small dimer,
meaning the protein is comprised of two polypeptides; the sequences of the two
polypeptides are given in the table with single-letter abbreviations.
polypeptide sequences of ectatomin monomers
polypeptide
|protein sequence
polypeptide 1
GVIPKKIWETVCPTVEPWAKKCSGDIATYIKRECGKL
polypeptide 2
WSTIVKLTICPTLKSMAKKCEGSIATMIKKKCDK
You could use absorbance at 280nm or colorimetric assays like the Bradford assay or
bicinchoninic acid assay (BCA) assay to quantify the amount of ectatomin you have for
your experiment.
Why might you choose to use a colorimetric method instead of absorbance at 280nm?
Select the best answer.
a) Measuring absorbance at higher wavelengths, like 595nm or 562nm, is more accurate
than measuring absorbance at 280nm.
b) The Bradford…
Chapter 6 Solutions
Biochemistry: Concepts and Connections (2nd Edition)
Ch. 6 - Prob. 1PCh. 6 - Bovine pancreatic trypsin inhibitor (BPTI; Figure...Ch. 6 - A schematic structure of the subunit of...Ch. 6 - In the protein adenylate kinase, the C-terminal...Ch. 6 - Give two reasons to explain why a proline residue...Ch. 6 - Consider a small protein containing 101 amino acid...Ch. 6 - a. Based on a more conservative answer to Problem...Ch. 6 - The following sequence is part of a globular...Ch. 6 - a. A protein is found to be a tetramer of...Ch. 6 - Under physiological conditions, the protein...
Ch. 6 - Theoretical and experimental measurements show...Ch. 6 - The peptide hormone vasopressin is used in the...Ch. 6 - A protein gives under conditions of buffer...Ch. 6 - A protein gives a single band on SDS get...Ch. 6 - It has been postulated that the normal...Ch. 6 - Below are shown two views of the backbone...Ch. 6 - Do you expect a Pro Gly mutation in a...Ch. 6 - Rank the following in terms of predicted rates...Ch. 6 - Shown below are two cartoon views of the small...Ch. 6 - Prob. 20PCh. 6 - In most cases, mutations in the core of protein...Ch. 6 - A Leu Ala mutation at a site buried the core of...Ch. 6 - Disulfide bonds have been shown to stabilize...Ch. 6 - Cartoon renderings of the proteins Top 7 and adaH2...
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