Biochemistry: Concepts and Connections (2nd Edition)
Biochemistry: Concepts and Connections (2nd Edition)
2nd Edition
ISBN: 9780134641621
Author: Dean R. Appling, Spencer J. Anthony-Cahill, Christopher K. Mathews
Publisher: PEARSON
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Chapter 6, Problem 13P

A protein gives under conditions of buffer composition, pH, and temperature that are close to physiological conditions, a molecular weight by size exclusion measurements of 140,000 g/mol. When the same protein studied by SDS gel electrophoresis in the absence or presence of the reducing agent β -mercaptoethanol (BME), the patterns seen, respectively, in lanes A and B areobserved Lane C contains standards of molecular weight indicated. From these data, describe the native protein, in terms of the kinds of subunits present the stoichiometry of subunits, and the kinds of bonding (covalent, noncovalent) existing between subunits
Chapter 6, Problem 13P, A protein gives under conditions of buffer composition, pH, and temperature that are close to

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a) What are the differences between the Direct & Indirect Immunofluorescence Assays? (0.5 mark) b) What are the advantages of the Indirect Immunofluorescence Assays? (0.5 mark) c) A Super-Resolution Imaging Technique was developed in 2018 using imidazole, a His-tag ligand conjugated with a fluorophore to report the presence of a recombinant His-tag protein target, (Sci Rep, 2018, 8:5507). How does this technique improve the image quality? (2 marks)
a) What are the differences between the Direct & Indirect Immunofluorescence Assays?  b) What are the advantages of the Indirect Immunofluorescence Assays?  c) A Super-Resolution Imaging Technique was developed in 2018 using imidazole, a His-tag ligand conjugated with a fluorophore to report the presence of a recombinant His-tag protein target, (Sci Rep, 2018, 8:5507). How does this technique improve the image quality?
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Biomolecules - Protein - Amino acids; Author: Tutorials Point (India) Ltd.;https://www.youtube.com/watch?v=ySNVPDHJ0ek;License: Standard YouTube License, CC-BY