Genetics: From Genes to Genomes, 5th edition
Genetics: From Genes to Genomes, 5th edition
5th Edition
ISBN: 9780073525310
Author: Leland H. Hartwell, Michael L. Goldberg, Janice A. Fischer, Leroy Hood, Charles F. Aquadro
Publisher: McGraw-Hill Education
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Chapter 6, Problem 10P

When a double-stranded DNA molecule is exposed to high temperature, the two strands separate, and the molecule loses its helical form. We say the DNA has been denatured. (Denaturation also occurs when DNA is exposed to acid or alkaline solutions.)

a. Regions of the DNA that contain many A–T base pairs are the first to become denatured as the temperature of a DNA solution is raised. Thinking about the chemical structure of the DNA molecule, why do you think the A–T-rich regions denature first?
b. If the temperature is lowered, the original DNA strands can reanneal, or renature. In addition to the full double-stranded molecules, some molecules of the type shown here are seen when the molecules are examined under the electron microscope. How can you explain these structures?

Chapter 6, Problem 10P, When a double-stranded DNA molecule is exposed to high temperature, the two strands separate, and

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