Genetics: From Genes to Genomes, 5th edition
Genetics: From Genes to Genomes, 5th edition
5th Edition
ISBN: 9780073525310
Author: Leland H. Hartwell, Michael L. Goldberg, Janice A. Fischer, Leroy Hood, Charles F. Aquadro
Publisher: McGraw-Hill Education
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Chapter 6, Problem 16P

In Meselson and Stahl’s density shift experiments (diagrammed in Fig. 6.20 on p. 189), describe the results you would expect in each of the following situations:

a. Conservative replication after two rounds of DNA synthesis on 14N.
b. Semiconservative replication after three rounds of DNA synthesis on 14N.
c. Dispersive replication after three rounds of DNA synthesis on 14N.
d. Conservative replication after three rounds of DNA synthesis on 14N.
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Draw a rough sketch of the control and experimental data using a normal dose/response curve plot: i.e. % of total bound ligand vs. concentration. Indicate Kd and Bmax on this sketch. You don’t need to use exact numbers, but the relative proportions should be close
Name something that could be happening to glutamate transporters that could have this effect on glutamate transport (i.e. the difference in glutamate transporters between control and experimental conditions). (Bonus: if you can also guess what the experimental condition is).
a. For the control condition, calculate Kd. Include units and show your work. b. For the control condition, calculate Bmax. Include units and show your work.  c. For the experimental condition, calculate Kd. Include units and show your work. For the experimental condition, calculate Bmax. Include units and show your work.

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Genetics: From Genes to Genomes, 5th edition

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