Brock Biology of Microorganisms (15th Edition)
15th Edition
ISBN: 9780134261928
Author: Michael T. Madigan, Kelly S. Bender, Daniel H. Buckley, W. Matthew Sattley, David A. Stahl
Publisher: PEARSON
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Chapter 5.5, Problem 3MQ
What is meant by the word sterile? Why is aseptic technique necessary for successful maintenance of pure cultures in the laboratory?
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Chapter 5 Solutions
Brock Biology of Microorganisms (15th Edition)
Ch. 5.1 - Define the term generation. What is meant by the...Ch. 5.1 - How do binary fission and budding cell division...Ch. 5.1 - How does the biofilm growth mode differ from that...Ch. 5.1 - Prob. 1CRCh. 5.2 - What is a semilogarithmic plot and what...Ch. 5.2 - For an exponentially growing culture that...Ch. 5.2 - For testing a bacteriums response to a toxic...Ch. 5.2 - How is the generation time (g) of an exponentially...Ch. 5.3 - In which phase of the growth curve do cells divide...Ch. 5.3 - Prob. 2MQ
Ch. 5.3 - Prob. 3MQCh. 5.3 - Describe the growth cycle of a population of...Ch. 5.4 - How do microorganisms in a chemostat differ from...Ch. 5.4 - What happens in a chemostat if the dilution rate...Ch. 5.4 - Do pure cultures have to be used in a chemostat?Ch. 5.4 - How does a chemostat regulate growth rate and cell...Ch. 5.5 - Why would a complex culture medium for Leuconostoc...Ch. 5.5 - In which medium shown in Table 5.1, defined or...Ch. 5.5 - What is meant by the word sterile? Why is aseptic...Ch. 5.5 - How many cells could be present in a single...Ch. 5.5 - Prob. 1CRCh. 5.6 - What are some of the problems that can arise when...Ch. 5.6 - Using microscopic techniques, how could you tell...Ch. 5.6 - Are total cell counts useful if one does not know...Ch. 5.7 - Why is a viable count more sensitive than a...Ch. 5.7 - Describe how you would dilute a bacterial culture...Ch. 5.7 - Prob. 3MQCh. 5.7 - How does a viable count differ from a total count?Ch. 5.8 - List two advantages of using turbidity as a...Ch. 5.8 - Describe how you could use a turbidity measurement...Ch. 5.8 - How can turbidity be used as a measure of cell...Ch. 5.9 - How does a hyperthermophile differ from a...Ch. 5.9 - Prob. 2MQCh. 5.9 - E. coli can grow at a higher temperature in a...Ch. 5.9 - Examine the graph in Figure 5.17. Why is the...Ch. 5.10 - Prob. 1MQCh. 5.10 - What molecular adaptations to cold temperatures...Ch. 5.10 - Prob. 1CRCh. 5.11 - Which phylogenetic domain includes species with...Ch. 5.11 - How does the membrane structure of...Ch. 5.11 - What is Taq polymerase and why is it important?Ch. 5.11 - How do cells of hyperthermophiles prevent heat...Ch. 5.12 - How does the concentration of H+ change when a...Ch. 5.12 - What terms are used to describe organisms whose...Ch. 5.12 - Prob. 3MQCh. 5.12 - Concerning the pH of the environment and of the...Ch. 5.13 - What is the aw of pure water? What is the lower...Ch. 5.13 - What are compatible solutes, and when and why are...Ch. 5.13 - How does a halophile maintain positive water...Ch. 5.14 - How does an obligate aerobe differ from a...Ch. 5.14 - How does a reducing agent work? Give an example of...Ch. 5.14 - How does Superoxide dismutase or superoxide...Ch. 5.14 - Contrast an aerotolerant and an obligate anaerobe...Ch. 5.15 - Why is heat an effective sterilizing agent?Ch. 5.15 - What steps are necessary to ensure the sterility...Ch. 5.15 - Distinguish between the sterilization of...Ch. 5.15 - Contrast the terms thermal death time and decimal...Ch. 5.16 - Define D10 and explain why the killing dose for...Ch. 5.16 - Prob. 2MQCh. 5.16 - Prob. 3MQCh. 5.16 - Prob. 1CRCh. 5.17 - Distinguish between the antimicrobial effects of...Ch. 5.17 - Describe how the minimum inhibitory concentration...Ch. 5.17 - Distinguish between a sterilant, a disinfectant,...Ch. 5.17 - Describe the procedure for obtaining the minimum...Ch. 5 - A medium was inoculated with 5 106 cells/ml of...Ch. 5 - Escherichia coli but not Pyrolobus fumarii will...Ch. 5 - In which direction (into or out of the cell) will...
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