BIOLOGY (LOOSELEAF)-W/CONNECT
12th Edition
ISBN: 9781260692181
Author: Raven
Publisher: MCG
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Chapter 5.4, Problem 2LO
Summary Introduction
To differentiate: Between channel proteins and carrier proteins.
Introduction: A cell membrane is also called a cytoplasmic membrane or plasma membrane. The major components of the cell membrane are glycolipids, phospholipids, cholesterol, and proteins.
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a) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
b) Using the equation above, calculate the transformation efficiency.
c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
Chapter 5 Solutions
BIOLOGY (LOOSELEAF)-W/CONNECT
Ch. 5.1 - Prob. 1LOCh. 5.1 - Explain the fluid mosaic model of membrane...Ch. 5.2 - List the different components of phospholipids.Ch. 5.2 - Prob. 2LOCh. 5.2 - Prob. 3LOCh. 5.3 - Illustrate the functions of membrane proteins.Ch. 5.3 - Prob. 2LOCh. 5.3 - Prob. 3LOCh. 5.4 - Compare simple diffusion and facilitated...Ch. 5.4 - Prob. 2LO
Ch. 5.4 - Prob. 3LOCh. 5.5 - Prob. 1LOCh. 5.5 - Prob. 2LOCh. 5.5 - Prob. 3LOCh. 5.6 - Distinguish between endocytosis and exocytosis.Ch. 5.6 - Illustrate how endocytosis can be specific.Ch. 5 - According to the fluid mosaic model, membranes are...Ch. 5 - Prob. 2IQCh. 5 - The fluid mosaic model of the membrane describes...Ch. 5 - What chemical property characterizes the interior...Ch. 5 - The transmembrane domain of an integral membrane...Ch. 5 - The specific function of a membrane within a cell...Ch. 5 - The movement of water across a membrane is...Ch. 5 - Prob. 6UCh. 5 - Which of the following is NOT a mechanism for...Ch. 5 - A bacterial cell that can alter the composition of...Ch. 5 - What variable(s) influence(s) whether a nonpolar...Ch. 5 - Which of the following does NOT contribute to the...Ch. 5 - How are active transport and coupled transport...Ch. 5 - A cell can use the process of facilitated...Ch. 5 - figure 5.5 describes a classic experiment...Ch. 5 - Each compartment of the endomembrane system of a...Ch. 5 - The distribution of lipids in the ER membrane is...
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- Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…arrow_forwardOverview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…arrow_forwardBased on your results, which suspect's DNA best matches the DNA found at the crime scene?arrow_forward
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