BIOLOGY (LOOSELEAF)-W/CONNECT
12th Edition
ISBN: 9781260692181
Author: Raven
Publisher: MCG
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Chapter 5.1, Problem 1LO
Summary Introduction
To describe: The components of the biological membrane.
Introduction: The cell membrane is also called a cytoplasmic membrane or plasma membrane. The cell membrane a biological membrane. It separated the interior of all cells from outside environments and it protects the cell from its surroundings.
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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 5 Solutions
BIOLOGY (LOOSELEAF)-W/CONNECT
Ch. 5.1 - Prob. 1LOCh. 5.1 - Explain the fluid mosaic model of membrane...Ch. 5.2 - List the different components of phospholipids.Ch. 5.2 - Prob. 2LOCh. 5.2 - Prob. 3LOCh. 5.3 - Illustrate the functions of membrane proteins.Ch. 5.3 - Prob. 2LOCh. 5.3 - Prob. 3LOCh. 5.4 - Compare simple diffusion and facilitated...Ch. 5.4 - Prob. 2LO
Ch. 5.4 - Prob. 3LOCh. 5.5 - Prob. 1LOCh. 5.5 - Prob. 2LOCh. 5.5 - Prob. 3LOCh. 5.6 - Distinguish between endocytosis and exocytosis.Ch. 5.6 - Illustrate how endocytosis can be specific.Ch. 5 - According to the fluid mosaic model, membranes are...Ch. 5 - Prob. 2IQCh. 5 - The fluid mosaic model of the membrane describes...Ch. 5 - What chemical property characterizes the interior...Ch. 5 - The transmembrane domain of an integral membrane...Ch. 5 - The specific function of a membrane within a cell...Ch. 5 - The movement of water across a membrane is...Ch. 5 - Prob. 6UCh. 5 - Which of the following is NOT a mechanism for...Ch. 5 - A bacterial cell that can alter the composition of...Ch. 5 - What variable(s) influence(s) whether a nonpolar...Ch. 5 - Which of the following does NOT contribute to the...Ch. 5 - How are active transport and coupled transport...Ch. 5 - A cell can use the process of facilitated...Ch. 5 - figure 5.5 describes a classic experiment...Ch. 5 - Each compartment of the endomembrane system of a...Ch. 5 - The distribution of lipids in the ER membrane is...
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