The factors involved in membrane fluidity. Introduction: The cell membrane is also called a cytoplasmic membrane or plasma membrane. The major components of the cell membrane are glycolipids, phospholipids, cholesterol, and protiens.

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Chapter 5.2, Problem 3LO

Summary Introduction

To describe: The factors involved in membrane fluidity.

Introduction: The cell membrane is also called a cytoplasmic membrane or plasma membrane. The major components of the cell membrane are glycolipids, phospholipids, cholesterol, and protiens.

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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results? 2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab? 3) Why are the cells incubated at 42°C?

Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…

Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…

Answer 2

Question

Chapter 5.2, Problem 3LO

Summary Introduction

To describe: The factors involved in membrane fluidity.

Introduction: The cell membrane is also called a cytoplasmic membrane or plasma membrane. The major components of the cell membrane are glycolipids, phospholipids, cholesterol, and protiens.

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Answer 3

Question

Chapter 5.2, Problem 3LO

Summary Introduction

To describe: The factors involved in membrane fluidity.

Introduction: The cell membrane is also called a cytoplasmic membrane or plasma membrane. The major components of the cell membrane are glycolipids, phospholipids, cholesterol, and protiens.

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Answer 4

Question

Chapter 5.2, Problem 3LO

Summary Introduction

To describe: The factors involved in membrane fluidity.

Introduction: The cell membrane is also called a cytoplasmic membrane or plasma membrane. The major components of the cell membrane are glycolipids, phospholipids, cholesterol, and protiens.

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Answer 5

Chapter 5.2, Problem 3LO

Summary Introduction

To describe: The factors involved in membrane fluidity.

Introduction: The cell membrane is also called a cytoplasmic membrane or plasma membrane. The major components of the cell membrane are glycolipids, phospholipids, cholesterol, and protiens.

Answer 6

Chapter 5.2, Problem 3LO

Summary Introduction

To describe: The factors involved in membrane fluidity.

Introduction: The cell membrane is also called a cytoplasmic membrane or plasma membrane. The major components of the cell membrane are glycolipids, phospholipids, cholesterol, and protiens.

Answer 7

Chapter 5.2, Problem 3LO

Summary Introduction

To describe: The factors involved in membrane fluidity.

Introduction: The cell membrane is also called a cytoplasmic membrane or plasma membrane. The major components of the cell membrane are glycolipids, phospholipids, cholesterol, and protiens.

Answer 8

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Ch. 5.1 - Prob. 1LO Ch. 5.1 - Explain the fluid mosaic model of membrane...Ch. 5.2 - List the different components of phospholipids.Ch. 5.2 - Prob. 2LO Ch. 5.2 - Prob. 3LO Ch. 5.3 - Illustrate the functions of membrane proteins.Ch. 5.3 - Prob. 2LO Ch. 5.3 - Prob. 3LO Ch. 5.4 - Compare simple diffusion and facilitated...Ch. 5.4 - Prob. 2LO

The factors involved in membrane fluidity. Introduction: The cell membrane is also called a cytoplasmic membrane or plasma membrane. The major components of the cell membrane are glycolipids, phospholipids, cholesterol, and protiens.

Solution Summary: The author explains that the cell membrane is a cytoplasmic membrane or plasma membrane. The degree of membrane fluidity alters with the composition of the membrane itself.

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To describe: The factors involved in membrane fluidity.

Introduction: The cell membrane is also called a cytoplasmic membrane or plasma membrane. The major components of the cell membrane are glycolipids, phospholipids, cholesterol, and protiens.

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Chapter 5 Solutions

The factors involved in membrane fluidity. Introduction: The cell membrane is also called a cytoplasmic membrane or plasma membrane. The major components of the cell membrane are glycolipids, phospholipids, cholesterol, and protiens.

Solution Summary: The author explains that the cell membrane is a cytoplasmic membrane or plasma membrane. The degree of membrane fluidity alters with the composition of the membrane itself.

Concept explainers

Videos

To describe: The factors involved in membrane fluidity. Introduction: The cell membrane is also called a cytoplasmic membrane or plasma membrane. The major components of the cell membrane are glycolipids, phospholipids, cholesterol, and protiens.

Want to see the full answer?

Chapter 5 Solutions

To describe: The factors involved in membrane fluidity.

Introduction: The cell membrane is also called a cytoplasmic membrane or plasma membrane. The major components of the cell membrane are glycolipids, phospholipids, cholesterol, and protiens.