1. Cultured cells were treated with growth factor EGF 2. Cell lysates were prepared, proteins separated by SDS-3. PAGE then subjected to WB using antibodies against Erk and phosphorylated form of Erk (p-Erk) p-Erk Erk 0 1 3 mM EGF What does the result show?

1. Cultured cells were treated with growth factor EGF 2. Cell lysates were prepared, proteins separated by SDS-3. PAGE then subjected to WB using antibodies against Erk and phosphorylated form of Erk (p-Erk) p-Erk Erk 0 1 3 mM EGF What does the result show?

Essentials of Pharmacology for Health Professions

7th Edition

ISBN:9781305441620

Author:WOODROW

Publisher:WOODROW

Chapter14: Antineoplastic Drugs

Section: Chapter Questions

Problem 10CRQ

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Question

Does it show the level of proteins? What about the amount? Levels of protein activation? How can you tell? Does the thickness tell you anything? What about the number of the lines?

1. Cultured cells were treated with growth factor EGF
2. Cell lysates were prepared, proteins separated by SDS-3.
PAGE then subjected to WB using antibodies against Erk and
phosphorylated form of Erk (p-Erk)
p-Erk
Erk
0 1 3 mM EGF
What does the result show?

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