Page < 5 28. (12 points) Growth factor EGF binds to EGF receptor (EGFR) on the cell surface. The binding initiates receptor-mediated endocytosis. You treated cells with EGF and collected cell lysates at 15, 30, 60, 120, and 180 minutes later. The cell lysates collected at different time points were subjected to Western blot analysis to detect EGFR. The result is shown in A. A EGF receptor (EGFR) + EGF 0 15 30 60 120 180 min > Chloroquine is a drug that prevent acidification of the lysosomal lumen. In your next experiment, you treated cells with Chloroquine first, then conducted the same experiment as described in A above (EGF treatment followed by Western blot analysis. The Western blot result is shown in B. e: B Chloroquine+ EGF 0 15 30 60 120 180 min EGF receptor (EGFR) Answer the following questions a. From the result shown in A, briefly explain the fate of EGFR in the cells following EGF stimulation b. From the result shown in B, briefly explain the impact of chloroquine on the fate of EGFR following EGF stimulation c. Wh ou think chloroquine (which prevents acidification of the lysosomal lumen) had such an impact on EGFR as you described above in b.?

Page < 5 28. (12 points) Growth factor EGF binds to EGF receptor (EGFR) on the cell surface. The binding initiates receptor-mediated endocytosis. You treated cells with EGF and collected cell lysates at 15, 30, 60, 120, and 180 minutes later. The cell lysates collected at different time points were subjected to Western blot analysis to detect EGFR. The result is shown in A. A EGF receptor (EGFR) + EGF 0 15 30 60 120 180 min > Chloroquine is a drug that prevent acidification of the lysosomal lumen. In your next experiment, you treated cells with Chloroquine first, then conducted the same experiment as described in A above (EGF treatment followed by Western blot analysis. The Western blot result is shown in B. e: B Chloroquine+ EGF 0 15 30 60 120 180 min EGF receptor (EGFR) Answer the following questions a. From the result shown in A, briefly explain the fate of EGFR in the cells following EGF stimulation b. From the result shown in B, briefly explain the impact of chloroquine on the fate of EGFR following EGF stimulation c. Wh ou think chloroquine (which prevents acidification of the lysosomal lumen) had such an impact on EGFR as you described above in b.?

Curren'S Math For Meds: Dosages & Sol

11th Edition

ISBN:9781305143531

Author:CURREN

Publisher:CURREN

Chapter10: Reconstitution Of Powdered Drugs

Section: Chapter Questions

Problem 3.3P

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Researchers measured prolactin and growth hormone in the blood plasma of six subjects at regular intervals over a 24-hour period. The investigators were interested in determining if the levels of these two hormones cycle over a 24-hour period. The Figure presents the results of their experiments, with the y axis values showing the amount of each hormone expressed as a percentage of the 24-hour mean value, and each point showing the average of the six subjects levels. FIGURE Mean concentrations of prolactin and growth hormone in plasma, averaged for six subjects and expressed as a percentage of the average concentrations measured for the 24-hour period. Do the results support the hypothesis that the changes in prolactin level over a 24-hour period are associated with the sleepwake cycle? Source: From J. F. Sassin et al. 1972. Human prolactin: 24-hour pattern with increased release during sleep. Science 177:12051207.
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