Laboratory Experiments in Microbiology (11th Edition)
11th Edition
ISBN: 9780321994936
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Textbook Question
Chapter 5, Problem 1CA
Bacteria can be seen without staining. Why, then, was Koch’s recommendation of fixing and staining important for the discovery of the bacterial causes of diseases?
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Chapter 5 Solutions
Laboratory Experiments in Microbiology (11th Edition)
Ch. 5 - Of what value is a simple stain?Ch. 5 - What is the purpose of heat-fixing the smear?Ch. 5 - Another method of fixing smears is to use methanol...Ch. 5 - In heat-fixing, what would happen if too much heat...Ch. 5 - Methylene blue can be prepared as a basic stain or...Ch. 5 - Prob. 2CTCh. 5 - Bacteria can be seen without staining. Why, then,...Ch. 5 - Quality control staff in a sterilization unit of a...
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- Which of the following events normally activates a GTP-binding protein? OA. GTP hydrolysis by the protein OB. Activation of an upstream GTPase-activating protein OC. Activation of an upstream guanine nucleotide exchange factor OD. Phosphorylation of a bound GDP molecule by an upstream phosphorylase OE. Pi release after GTP hydrolysisarrow_forwardProteins that are synthesized on the Rough ER are imported into the ER lumen after their signal sequence is recognized. Which of the following is responsible for the signal recognition? OA. Signal Recognition Particle, OB. Signal Sequence C. Stop-transfer sequence OD. Both B and C OE. None of the abovearrow_forwardStroma Light 4 H 4 H I Thylakoid- membran 2 H₂O D1 D2 Min Light 2,6 ATP 2 NADP+ 2 NADPH/H+ Fp 2,6 ADP +2,6 P 8 H+ 12 H+ 4 H+ Lumen PS II Cyt bef PS I ATP-Synthase 12345 5a 5b 6arrow_forward
- Which of the following statements is TRUE regarding the signal recognition particle (SRP)? OA. SRP is made up of RNA and Proteins B. SRP binds to the signal sequence OC. SRP binds to the elongation factor binding site on the ribosome and pauses translation D. All of the above are true statements OE. None of the above are true statementsarrow_forwardWhich of the following pathways does NOT directly deliver materials to lysosomes? OA. Endocytosis B. Exocytosis OC. Phagocytosis OD. Autophagy OE. Macropinocytosisarrow_forwardYou suspect a protein to be secreted out of a cell. What experiment would you perform to follow the movement of this protein through the secretory pathway? A. Pulse - Chase Experiment OB. Signal Sequence Deletion C. Signal Sequence Mutation OD. All of the above E. None of the abovearrow_forward
- A schematic drawing of the secretory and endocytic pathways is presented below. Indicate which component in the drawing (A to J) corresponds to each of the following. Your answer would be a 10-letter string composed of letters A to J only, c.g. HICDJABFGE. nuclear envelope Early endosome Late endosome ER Lysosome A ( ) cis Golgi cisterna () modial Golgi cisterna () trans Golgi cisterna [] cis Golgi network (CGN) () trans Golgi network (TGN) () Secretory Vesicle B C D E CYTOSOL F G H plasma membranearrow_forwardWhich of the following guide transport vesicles to their target membrane? A. Rab monomeric GTPases B. Signal Sequence OC. Heat Shock Proteins OD. All of the above O E. None of the abovearrow_forwardStroma Light 4 H 4 H I Thylakoid- membran 2 H₂O D1 D2 Min Light 2,6 ATP 2 NADP+ 2 NADPH/H+ Fp 2,6 ADP +2,6 P 8 H+ 12 H+ 4 H+ Lumen PS II Cyt bef PS I ATP-Synthase 12345 5a 5b 6arrow_forward
- Which of the following promote membrane fusion between the transport vesicle and the target compartment? OA. Endocytosis OB. t-SNARE OC. v-SNARE OD. Both B and C O E. None of the abovearrow_forwardThe following diagram depicts the topology of a multipass transmembrane protein in the endoplasmic reticulum (ER) membrane. Which set of helices act Cytosol N C 1 2 as stop-transfer signals in this protein? 2 3 4 5 6 OA 1,2,3 OB. 4. 5. 6 OC. 1.3.5 D. 2, 4, 6 OE1.6 ER lumenarrow_forwardA signal peptidase cleaves off the signal sequence upon import of the protein into the mitochondrial matrix. Where is this signal peptidase located? A. Outer membrane OB. Inner membrane C. Mitochondrial Matrix OD. A and B OE. None of the abovearrow_forward
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