Laboratory Experiments in Microbiology (11th Edition)
11th Edition
ISBN: 9780321994936
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Question
Chapter 5, Problem 2CT
Summary Introduction
To write:
Whether the dyes other than methylene blue can be utilized for direct staining.
Introduction:
Staining is a method that provides variance to the microscopic image for enhanced vision under the microscope. It is a method that is extensively used for the assessment of cells and tissue components. It is often used to draw attention to the microbial structures for viewing with the help of diverse microscopes.
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Is it possible to perform double or triple staining using fluorescent dyes? Explain.
Methylene blue can be prepared as a basic stain or an acidic stain. How would the pH of the stain affect
the staining of bacteria?
Can dyes other than methylene blue be used for direct staining? Briefly explain.
Explain the mechanism of staining for each of the stains used.
Iodine
Methyl green
Nigrosin
Chapter 5 Solutions
Laboratory Experiments in Microbiology (11th Edition)
Ch. 5 - Of what value is a simple stain?Ch. 5 - What is the purpose of heat-fixing the smear?Ch. 5 - Another method of fixing smears is to use methanol...Ch. 5 - In heat-fixing, what would happen if too much heat...Ch. 5 - Methylene blue can be prepared as a basic stain or...Ch. 5 - Prob. 2CTCh. 5 - Bacteria can be seen without staining. Why, then,...Ch. 5 - Quality control staff in a sterilization unit of a...
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- Briefly explain the difference between Wright Staining and H&E Staining (why use one over the other)arrow_forwardWhat is the purpose of acid-fast staining? What information can you gain from this procedure? Please explain.arrow_forwardWhy must heat or a surface-active agent be used with application of the primary stain during acid-fast staining?arrow_forward
- What are the pros and cons of using the TCE stain-free method versus a traditional gel staining method (CBB staining)?arrow_forwardDescribe how simple staining and fluorescence staining are similar and how they are different. What are the advantages of each?arrow_forwardWhat is the difference between Giemsa and Wright stain?arrow_forward
- Explain the importance of steam during the acid-fast staining procedure. Discuss the advantages of differential staining procedures over the simple staining techniques. List and briefly describe the functions/purpose of each of the different stains used in acid fast staining.arrow_forwardDefine fluorescent staining.arrow_forwardDuring staining, the first step is to dewax the slide. What is the purpose of the step? What is the effect of insufficient dewaxing?arrow_forward
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