Laboratory Experiments in Microbiology (11th Edition)
11th Edition
ISBN: 9780321994936
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Textbook Question
Chapter 5, Problem 1CT
Methylene blue can be prepared as a basic stain or an acidic stain. How would the pH of the Stain affect the staining of bacteria?
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Methylene blue can be prepared as a basic stain or an acidic stain. How would the pH of the stain affect
the staining of bacteria?
Can dyes other than methylene blue be used for direct staining? Briefly explain.
Crystal violet is an example of what type of stain?
For each stain, indicate if it’s a cationic dye or an anionic dye (2 pts).
Stain
Cationic or Anionic Dye
malachite green
crystal violet
nigrosin
safranin
Chapter 5 Solutions
Laboratory Experiments in Microbiology (11th Edition)
Ch. 5 - Of what value is a simple stain?Ch. 5 - What is the purpose of heat-fixing the smear?Ch. 5 - Another method of fixing smears is to use methanol...Ch. 5 - In heat-fixing, what would happen if too much heat...Ch. 5 - Methylene blue can be prepared as a basic stain or...Ch. 5 - Prob. 2CTCh. 5 - Bacteria can be seen without staining. Why, then,...Ch. 5 - Quality control staff in a sterilization unit of a...
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- Both crystal violet and safranin are basic stains and may be used to do simple stains on Gram positive and negative cells. This being the case, explain how they end up staining Gram positive and gram negative cells differently.arrow_forwardExplain the mechanism of staining for each of the stains used. Iodine Methyl green Nigrosinarrow_forwardFor What purpose is Giemsa stain used for?arrow_forward
- Draw what you would expect to see for acid fast stain and endospore stain procedure if you did not stain with counterstain. Color pencils need to be used.arrow_forwardExplain why only gram-negative cells undergo decolorization during the gram staining procedure. Cite the purpose of each of the following reagents in a differential staining procedure. Primary stain Mordant Decolorizing Agent Counter stain What might happen if the Gram staining procedure is performed on a culture incubated for a little over a dayarrow_forwardChoose the dyes or reagents used in the Endospore stain. (More than one choices are correct) a acid alcohol b crystal violet c carbolfuschin d malachite green e safranin f iodine g alcohol h methylene bluearrow_forward
- The bacterial cells in the image below were stained with a high concentration of carbolfuschin for an extended period of time (5 minutes) while the slide was heated. The slide was decolorized with a mixture of acid and alcohol. The slide was then counter-stained with methylene blue. What kind of stain does this describe?arrow_forwardSelect all of the following that represent examples of positive stains. Check All That Apply nigrosin methylene blue crystal violet India ink safraninarrow_forwardAbout the Gram staining technique, explain: What is the purpose of using lugol and alcohol in this type of coloring?arrow_forward
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