Laboratory Experiments in Microbiology (11th Edition)
11th Edition
ISBN: 9780321994936
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Chapter 5, Problem 2Q
What is the purpose of heat-fixing the smear?
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What is the significance of heat fixing the smear?
Describe the procedure and purpose for making streak plate.
For preparation of a smear on a slide, what is the purpose of heat fixation? What problems can arise when the slide is heated in a flame?
Chapter 5 Solutions
Laboratory Experiments in Microbiology (11th Edition)
Ch. 5 - Of what value is a simple stain?Ch. 5 - What is the purpose of heat-fixing the smear?Ch. 5 - Another method of fixing smears is to use methanol...Ch. 5 - In heat-fixing, what would happen if too much heat...Ch. 5 - Methylene blue can be prepared as a basic stain or...Ch. 5 - Prob. 2CTCh. 5 - Bacteria can be seen without staining. Why, then,...Ch. 5 - Quality control staff in a sterilization unit of a...
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- How can the thickness of the smear affect the staining procedure?arrow_forwardWhy is it necessary to prepare both NSS and Iodine Smears?arrow_forwardIn preparing a bacterial smear for staining, heat fixation is done after the smear dries up.a.) Give the purpose of heat fixation.b.) What can be observed in wet mounts or hanging-drop slides that cannot be observed in heat-fixed slides?arrow_forward
- What is the purpose of a streak plate? Name another means of achieving the same purpose?arrow_forwardWhat is the pathogen that exhibits the greatest resistance to heat treatment is the bacteria ?arrow_forwardHow will the staining appear if if the destaining step were done at high temperature?arrow_forward
- Is it important to have a standardized way of counting and reporting AFB seen on a direct sputum smear? Why or why not?arrow_forwardWhat are the principle and basic concepts of SMEAR PREPARATION? (please explain it thoroughly in a lengthy but clear explanation, thank you so much.)arrow_forwardWhat is the chemical basis of the Xanthoproteic Test? Give the chemical reaction involved.arrow_forward
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