Laboratory Experiments in Microbiology (11th Edition)
11th Edition
ISBN: 9780321994936
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Textbook Question
Chapter 5, Problem 3Q
Another method of fixing smears is to use methanol instead of heat. How does alcohol chemically fix the bacteria?
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In the preparation of a bacterial smear, why is there a need to fix the bacteria to the slide? Aside from passing the slide over a flame, what are the other ways of fixing the bacteria to the slide?
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Chapter 5 Solutions
Laboratory Experiments in Microbiology (11th Edition)
Ch. 5 - Of what value is a simple stain?Ch. 5 - What is the purpose of heat-fixing the smear?Ch. 5 - Another method of fixing smears is to use methanol...Ch. 5 - In heat-fixing, what would happen if too much heat...Ch. 5 - Methylene blue can be prepared as a basic stain or...Ch. 5 - Prob. 2CTCh. 5 - Bacteria can be seen without staining. Why, then,...Ch. 5 - Quality control staff in a sterilization unit of a...
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- Why is it best to use sterile distilled water in the preparation of microbial suspension and dry smears for slide preparation? What possible error could happen if a glass slide is reused for slide preparation?arrow_forwardWhat staining technique is used here? What is the primary stain, decolorizing agent, and counter stain of this technique? Do we heat fix in here? Describe the organism with the blue color. Describe the organism with the bright pink color. Name the genus of bacteria with bright pink.arrow_forwardWhich of the following is false?a) A high-level disinfectant cannot be used as a sterilant.b) Critical items must be sterilized before use.c) Low numbers of endospores may remain on semicritical items.d) Standard sterilization procedures do not destroy prions.e) Quaternary ammonium compounds can be used to disinfect food preparation surfaces.arrow_forward
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