Microbiology: A Systems Approach
Microbiology: A Systems Approach
4th Edition
ISBN: 9780073402437
Author: Marjorie Kelly Cowan Professor
Publisher: McGraw-Hill Education
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Chapter 5, Problem 10CTQ

a.

Summary Introduction

To determine: The organism that was isolated from the stool if it was a single celled organism.

Introduction:

Pathogens are disease causing microbes, which maintain a parasitic relationship with the host by extracting the nutrient from the host, and damaging the other mechanisms within the body. Both unicellular and multicellular organisms act as pathogens. Unicellular organisms consist of protozoa, bacteria, unicellular fungi and algae.

b.

Summary Introduction

To determine:  The kingdom to which the organism, isolated from the stool of infected person belongs if it is unicellular and flagellated.

Introduction:

The flagellum is a hair like structure that allows a cell to move. They are present in all the three domains of the living world: bacteria, archaea, and eukaryotes. Specialized flagella in some organisms’ flagella are also used as sensory organelles that can sense changes in.

c.

Summary Introduction

To determine:  The kingdom to which the organism isolated from the stool of infected person belongs if cysts were found in the stool sample as well as in the well water.

Introduction:

The presence of cyst is an important characteristic test for determining the organism responsible for infection. The cysts are the parasites that are present inside the hard coating which prevent the organism during harsh conditions.

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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?   2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?   3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol   -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes.   Questions: 1) What differences would you expect to see between the…
Overview of Transformation Protocol   -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes.   Questions: 1)What is the selectable marker in this experiment? How…

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Microbiology: A Systems Approach

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