Campbell Biology In Focus, Loose-leaf Edition (3rd Edition)
3rd Edition
ISBN: 9780134895727
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky
Publisher: PEARSON
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Chapter 4.7, Problem 3CC
MAKE CONNECTIONS The polypeptide chain that makes up a tight junction weaves back and forth through the membrane four times, with two extracellular loops, and one loop plus short C-terminal and N-terminal tails in the cytoplasm. Looking at Figure 3.18, whatWhat would you predict about the amino acids making up the tight junction protein?
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25. Your friend works in a cell biology research lab. She is working
she calls p125, because its molecular mass is 125 kiloDaltons. She knows that
p125 is a transmembrane protein with three membrane-spanning domains. It has
been previously reported that p125 interacts with three other proteins called p175,
p80, and p50 (again,
polyacrylamide gel). These four proteins
in the cell. To determine how these proteins interact with the membrane, you
perform a set of experiments in which you first lyse the cells and save some of
your lysate, which you run in the input lane (labeled "I" in Figure Q25 below).
The lysate is then subjected to a low-speed centrifugation so that you separate out
the membrane fraction (which ends up in the pellet, "P") from the cytoplasm
(which is in the supernatant, "S"). You then wash the pellet from the first
extraction with a high-salt wash that does not disrupt the lipid bilayer, and save a
little bit to run on the gel. After the high-salt wash, you centrifuge…
Match each description with the type of transmembrane protein
Drawn below is a schematic of a transmembrane protein.
Extracellular
Cell
membrane
Cytosolic side
(a) From the list below, select the amino acid(s) that might by more common in the extracellular domain of
this membrane protein and whose side- chain can form hydrogen bonds with the surrounding water
molecules. Explain why you selected this option(s).
Lysine
Serine
Phenylalanine
Methionine
(b) From the list below, select the amino acid(s) that would likely be found in the transmembrane/
membrane spanning domain of this protein and whose side- chain interacts with the lipid bilayer.
Lysine
Serine
Phenylalanine
Methionine
Chapter 4 Solutions
Campbell Biology In Focus, Loose-leaf Edition (3rd Edition)
Ch. 4.1 - Prob. 1CCCh. 4.1 - Prob. 2CCCh. 4.2 - Briefly describe the structure and function of the...Ch. 4.2 - Prob. 2CCCh. 4.3 - What role do ribosomes play in carrying out...Ch. 4.3 - Describe the molecular composition of nucleoli,...Ch. 4.3 - WHAT IF? As a cell begins the process of dividing,...Ch. 4.4 - Describe the structural and functional...Ch. 4.4 - Describe how transport vesicles integrate the...Ch. 4.4 - WHAT IF? Imagine a protein that functions in the...
Ch. 4.5 - Describe two characteristics shared by...Ch. 4.5 - Prob. 2CCCh. 4.5 - Prob. 3CCCh. 4.6 - Prob. 1CCCh. 4.6 - WHAT IF? Males afflicted with Kartageners syndrome...Ch. 4.7 - In what way are the cells of plants and animals...Ch. 4.7 - Prob. 2CCCh. 4.7 - MAKE CONNECTIONS The polypeptide chain that makes...Ch. 4.8 - Colpidium colpoda is a unicellular protist that...Ch. 4 - Which structure is not part of the endomembrane...Ch. 4 - Which structure is common to plant and animal...Ch. 4 - Which of the following is present in a prokaryotic...Ch. 4 - Cyanide binds to at least one molecule involved in...Ch. 4 - Which cell would be best for studying lysosomes?...Ch. 4 - DRAW IT From memory, draw two eukaryotic cells....Ch. 4 - SCIENTIFIC INQUIRY In studying micrographs of an...Ch. 4 - FOCUS ON EVOLUTION Compare different aspects of...Ch. 4 - FOCUS ON ORGANIZATION Considering some of the...Ch. 4 - Prob. 10TYU
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