Brock Biology of Microorganisms (14th Edition)
14th Edition
ISBN: 9780321897398
Author: Michael T. Madigan, John M. Martinko, Kelly S. Bender, Daniel H. Buckley, David A. Stahl, Thomas Brock
Publisher: PEARSON
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Chapter 4.3, Problem 3MQ
- Approximately how large is the Escherichia coli genome in base pairs? How many genes does it contain?
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Approximately how large is the Escherichia coli genome in basepairs? How many genes does it contain?
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what is the size mm (bx x 0.3 x 0.000001), how many base pairs does it have, how many encoding segments or genes, how many Non-encoding segements or pseudogenes, and how many structural RNAs?
Chapter 4 Solutions
Brock Biology of Microorganisms (14th Edition)
Ch. 4.1 - Prob. 1MQCh. 4.1 - Prob. 2MQCh. 4.1 - Prob. 3MQCh. 4.1 - Prob. 4MQCh. 4.2 - Prob. 1MQCh. 4.2 - Prob. 2MQCh. 4.2 - Prob. 3MQCh. 4.2 - Why is supercoiling essential to a bacterial cell?...Ch. 4.3 - Prob. 1MQCh. 4.3 - Prob. 2MQ
Ch. 4.3 - Approximately how large is the Escherichia coli...Ch. 4.3 - Prob. 4MQCh. 4.4 - To which end (5 or 3) of a newly synthesized...Ch. 4.4 - Prob. 2MQCh. 4.5 - Prob. 1MQCh. 4.5 - Prob. 2MQCh. 4.5 - Prob. 3MQCh. 4.6 - Prob. 1MQCh. 4.6 - Prob. 2MQCh. 4.6 - Prob. 3MQCh. 4.7 - Prob. 1MQCh. 4.7 - Prob. 2MQCh. 4.7 - Prob. 3MQCh. 4.7 - Prob. 4MQCh. 4.8 - Prob. 1MQCh. 4.8 - Prob. 2MQCh. 4.8 - Prob. 3MQCh. 4.9 - What three major components make up an archaeal...Ch. 4.9 - Prob. 2MQCh. 4.9 - Prob. 3MQCh. 4.10 - Prob. 1MQCh. 4.10 - Prob. 2MQCh. 4.10 - Prob. 3MQCh. 4.11 - Prob. 1MQCh. 4.11 - Prob. 2MQCh. 4.11 - Prob. 3MQCh. 4.12 - Prob. 1MQCh. 4.12 - What is the function of the acceptor stem of a...Ch. 4.13 - What are the components of a ribosome? What...Ch. 4.13 - How is a completed polypeptide chain released from...Ch. 4.13 - How does tmRNA free stalled ribosomes?Ch. 4.14 - Prob. 1MQCh. 4.14 - Prob. 2MQCh. 4.14 - Prob. 3MQCh. 4.14 - Prob. 4MQCh. 4 - Prob. 1RQCh. 4 - Prob. 2RQCh. 4 - Prob. 3RQCh. 4 - Prob. 4RQCh. 4 - Prob. 5RQCh. 4 - Prob. 6RQCh. 4 - Prob. 7RQCh. 4 - Prob. 8RQCh. 4 - Why are errors in DNA replication so rare? What...Ch. 4 - Prob. 10RQCh. 4 - Prob. 11RQCh. 4 - Prob. 12RQCh. 4 - REVIEW QUESTIONS
13. Why do eukaryotic mRNAs have...Ch. 4 - Prob. 14RQCh. 4 - Prob. 15RQCh. 4 - Prob. 16RQCh. 4 - The enzyme activity that forms peptide bonds on...Ch. 4 - Define the types of protein structure: primary,...Ch. 4 - Prob. 19RQCh. 4 - Prob. 20RQCh. 4 - Prob. 1AQCh. 4 - Compare and contrast the activity of DNA and RNA...Ch. 4 - Prob. 3AQCh. 4 - In Chapter 10 we will learn about mutations,...
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- Recombinant protein production by a genetically modified Escherichia coli strain is proportional to cell growth. Ammonia is used as a nitrogen source for aerobic glucose respiration. The recombinant protein has the general formula CH1,55O0,31N0,25, while that of the cellular biomass is CH1,77O0,49N0,24. The biomass yield from glucose equals 0.50 g/g, while the recombinant protein yield from glucose corresponds to 20% of the cell yield from substrate.a) How much ammonia is required? What is the oxygen demand? (b) If the biomass yield remains the same, what are the ammonia and oxygen requirements for a wild-type strain of E. coli, with cell biomass of the same elemental composition, but unable to synthesize the recombinant protein? (c) On an industrial scale, cultivation takes place in a continuous fermenter at 28°C and the desired recombinant protein production rate is 7 g/h. Since the viscosity of the culture broth is considerable, the energy input due to agitation cannot be neglected.…arrow_forwardYou have been tasked with designing a new computer algorithm to identify protein-coding genes in bacterial genomes.a) Name 3 specific criteria/patterns you will use to define whether a given sequence could contain a full-length protein-coding gene, and briefly justify your choices.b) Do you think your algorithm will work well to detect proteins encoded by the archaean Sulfolobus acidocaldarius? Why or why not?c) Do you think your algorithm will work well to detect proteins encoded in the eukaryotic yeast Saccharomyces cerevisiae? Why or why not?arrow_forwardwhat are the genotypes of the following e.coli strains 1-4?arrow_forward
- The DNA of a deletion of alpha bacteriophage has a length of 15 micrometers instead of 17 micrometers? How many base pairs are missing from this mutant?arrow_forwardThe 13-Mb genome of the green alga Ostreococcus tauri contains ∼8000 genes. Compare the gene density in this eukaryote to that of E. coli (∼4300 genes) and that of A. thaliana (∼25,500 genes).arrow_forwardOn the gel diagram below, show how you believe these fragments will sort out during electrophoresis. Label each fragment with its correct number of base pairs. (8 fragments)arrow_forward
- how are okazaki fragments created?arrow_forwardhow will mutation (with - ) will affect E.coli grown in lactose medium. what is the implication of the following genotypes? i+ p+ o+ z- y+arrow_forwardThe presence (+) or absence (−) of six sequences in each of five bacterial artificial chromosome (BAC) clones (A–E) is indicated in the following table. Using these markers, put the BAC clones in their correct order and indicate the locations of the numbered sequences within them. Sequences BAC clone 1 2 3 4 5 6 A + − − − + − B − − − + − + C − + + − − − D − − + − + − E + − − + − −arrow_forward
- It is desired to isolate genomic DNA from liquid culture of S. cerevisiae yeast. A commercial kit will be used to isolate genomic DNA from this liquid culture. Answer the following questions to understand the strategy used by commercial kits for genomic DNA isolation. a) List all the steps from cell pellet preparation to DNA elution. b) With which feature can the membrane in the column that comes with the commercial kit bind DNA? c) Which component in the kit would you use to recover the DNA from the membrane of the column to which the DNA was attached?arrow_forwardWe have two specific strains of E. coli that have shown horizontal gene transfer (HGT) when mixed. To experimentally determine the method of HGT that is happening, the following conditions are set up in different tubes of culture media: A) Donor and recipient strain mixed together (control - no treatment). B) Donor and recipient strains mixed together, DNase added (can digest DNA in solution, not within cells).C) Special tube containing a membrane filter (with pores that allow DNA and viruses to pass through, but not bacterial cells) that separates two compartments. Donor strain is added on one side, the recipient strain on the other (they are separated by the filter).D) Donor and recipient strains mixed together, with chemical that inactivates viruses (chemical affects bacteriophages in solution so they are unable to attach to cells). The results: Tubes A, B, and D: HGT was observed. Tube C: HGT was NOT observed. Based on this, which type of HGT was occurring? Conjugation,…arrow_forwardHow many bits of information are stored in an 8-mer DNA sequence? In the E. coli genome? In the human genome?arrow_forward
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Genome Annotation, Sequence Conventions and Reading Frames; Author: Loren Launen;https://www.youtube.com/watch?v=MWvYgGyqVys;License: Standard Youtube License