Brock Biology of Microorganisms (14th Edition)
14th Edition
ISBN: 9780321897398
Author: Michael T. Madigan, John M. Martinko, Kelly S. Bender, Daniel H. Buckley, David A. Stahl, Thomas Brock
Publisher: PEARSON
expand_more
expand_more
format_list_bulleted
Concept explainers
Videos
Textbook Question
Chapter 4.2, Problem 4MQ
- Why is supercoiling essential to a bacterial cell? What enzyme facilitates this process?
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
What is the purpose of bacterial transformation?
What is the process of transformation in bacteria?
It is desired to isolate genomic DNA from liquid culture of S. cerevisiae yeast. A commercial kit will be used to isolate genomic DNA from this liquid culture. Answer the following questions to understand the strategy used by commercial kits for genomic DNA isolation.
a) List all the steps from cell pellet preparation to DNA elution.
b) With which feature can the membrane in the column that comes with the commercial kit bind DNA?
c) Which component in the kit would you use to recover the DNA from the membrane of the column to which the DNA was attached?
Chapter 4 Solutions
Brock Biology of Microorganisms (14th Edition)
Ch. 4.1 - Prob. 1MQCh. 4.1 - Prob. 2MQCh. 4.1 - Prob. 3MQCh. 4.1 - Prob. 4MQCh. 4.2 - Prob. 1MQCh. 4.2 - Prob. 2MQCh. 4.2 - Prob. 3MQCh. 4.2 - Why is supercoiling essential to a bacterial cell?...Ch. 4.3 - Prob. 1MQCh. 4.3 - Prob. 2MQ
Ch. 4.3 - Approximately how large is the Escherichia coli...Ch. 4.3 - Prob. 4MQCh. 4.4 - To which end (5 or 3) of a newly synthesized...Ch. 4.4 - Prob. 2MQCh. 4.5 - Prob. 1MQCh. 4.5 - Prob. 2MQCh. 4.5 - Prob. 3MQCh. 4.6 - Prob. 1MQCh. 4.6 - Prob. 2MQCh. 4.6 - Prob. 3MQCh. 4.7 - Prob. 1MQCh. 4.7 - Prob. 2MQCh. 4.7 - Prob. 3MQCh. 4.7 - Prob. 4MQCh. 4.8 - Prob. 1MQCh. 4.8 - Prob. 2MQCh. 4.8 - Prob. 3MQCh. 4.9 - What three major components make up an archaeal...Ch. 4.9 - Prob. 2MQCh. 4.9 - Prob. 3MQCh. 4.10 - Prob. 1MQCh. 4.10 - Prob. 2MQCh. 4.10 - Prob. 3MQCh. 4.11 - Prob. 1MQCh. 4.11 - Prob. 2MQCh. 4.11 - Prob. 3MQCh. 4.12 - Prob. 1MQCh. 4.12 - What is the function of the acceptor stem of a...Ch. 4.13 - What are the components of a ribosome? What...Ch. 4.13 - How is a completed polypeptide chain released from...Ch. 4.13 - How does tmRNA free stalled ribosomes?Ch. 4.14 - Prob. 1MQCh. 4.14 - Prob. 2MQCh. 4.14 - Prob. 3MQCh. 4.14 - Prob. 4MQCh. 4 - Prob. 1RQCh. 4 - Prob. 2RQCh. 4 - Prob. 3RQCh. 4 - Prob. 4RQCh. 4 - Prob. 5RQCh. 4 - Prob. 6RQCh. 4 - Prob. 7RQCh. 4 - Prob. 8RQCh. 4 - Why are errors in DNA replication so rare? What...Ch. 4 - Prob. 10RQCh. 4 - Prob. 11RQCh. 4 - Prob. 12RQCh. 4 - REVIEW QUESTIONS
13. Why do eukaryotic mRNAs have...Ch. 4 - Prob. 14RQCh. 4 - Prob. 15RQCh. 4 - Prob. 16RQCh. 4 - The enzyme activity that forms peptide bonds on...Ch. 4 - Define the types of protein structure: primary,...Ch. 4 - Prob. 19RQCh. 4 - Prob. 20RQCh. 4 - Prob. 1AQCh. 4 - Compare and contrast the activity of DNA and RNA...Ch. 4 - Prob. 3AQCh. 4 - In Chapter 10 we will learn about mutations,...
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- What order should the steps be in for this culture method?arrow_forwardWhen using bacteria to produce a particular protein, why is it usually better to use the cDNA version of the gene rather than the genomic DNA version?arrow_forwardWhat is the function of conjugation reactions in the biotransformation process?arrow_forward
- Does bacterial transformation occur naturally?arrow_forwardWhat is gene cloning? What is bacterial transformation? What is the difference between the two different methods?arrow_forwardIdentify the most mistaken (wrong) choice: a) Transcription machinery and an enhancer can bind to the chromosome at the same time. b) Organic matters may interfere with heat treatment of bacterial growth control. ( c) Nitrocellulose can be used to filter out microorganisms from a liquid solution. d) Time to kill a bacterial culture is not proportional to the number of microbes in the culturearrow_forward
- Why do adult human cells (other than germ cells and stem cells) NOT express the enzyme telomerase? In other words what benefit does not having telomerase provide to these cells?arrow_forwardWhat is the essential component of a typical bacterial genome?arrow_forwardDoes bacterial transformation occur in nature?arrow_forward
- Bacterial systems serve as an excellent model to express proteins but has a disadvantage – what is that disadvantage? List the points to differentiate three classes of restriction enzymes? Give an example of restriction enzyme that has ability to generate blunt and cohesive ends after digestion of DNA?arrow_forwardWe have two specific strains of E. coli that have shown horizontal gene transfer (HGT) when mixed. To experimentally determine the method of HGT that is happening, the following conditions are set up in different tubes of culture media: A) Donor and recipient strain mixed together (control - no treatment). B) Donor and recipient strains mixed together, DNase added (can digest DNA in solution, not within cells).C) Special tube containing a membrane filter (with pores that allow DNA and viruses to pass through, but not bacterial cells) that separates two compartments. Donor strain is added on one side, the recipient strain on the other (they are separated by the filter).D) Donor and recipient strains mixed together, with chemical that inactivates viruses (chemical affects bacteriophages in solution so they are unable to attach to cells). The results: Tubes A, B, and D: HGT was observed. Tube C: HGT was NOT observed. Based on this, which type of HGT was occurring? Conjugation,…arrow_forwardWhat is isosterism and bioisostreism? How does this concept help in biotransformation of different compounds?arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Biology: The Dynamic Science (MindTap Course List)BiologyISBN:9781305389892Author:Peter J. Russell, Paul E. Hertz, Beverly McMillanPublisher:Cengage Learning
Biology: The Dynamic Science (MindTap Course List)
Biology
ISBN:9781305389892
Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher:Cengage Learning
genetic recombination strategies of bacteria CONJUGATION, TRANSDUCTION AND TRANSFORMATION; Author: Scientist Cindy;https://www.youtube.com/watch?v=_Va8FZJEl9A;License: Standard youtube license