Connect With Learnsmart Labs Online Access For Prescott's Microbiology
11th Edition
ISBN: 9781260408997
Author: Joanne Willey
Publisher: Mcgraw-hill Higher Education (us)
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Textbook Question
Chapter 42.4, Problem 1MI
Why are modular genes particularly suitable as platforms for the development of new natural products of industrial interest?
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To produce transgenic plants, plant tissue is exposed to Agrobacteriumtumefaciens and then grown in media containing kanamycin, carbenicillin, and plant growth hormones. Explain the purpose behind each of these three agents. What would happen if you left out the kanamycin?
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Chapter 42 Solutions
Connect With Learnsmart Labs Online Access For Prescott's Microbiology
Ch. 42.1 - Why are the cells first fed lactose and then...Ch. 42.1 - What critical limiting factors are used in the...Ch. 42.1 - Prob. 2CCCh. 42.1 - What kinds of molecular changes result from...Ch. 42.1 - Prob. 4CCCh. 42.2 - Why is there interest in developing alternatives...Ch. 42.2 - What types of microbes are good candidates for the...Ch. 42.3 - What is the objective of the scale-up process and...Ch. 42.3 - What parameters can be monitored in a modern,...Ch. 42.3 - Which of the following are secondary metabolites:...
Ch. 42.4 - Why are modular genes particularly suitable as...Ch. 42.4 - Prob. 2MICh. 42.4 - Prob. 1CCCh. 42.4 - Prob. 2CCCh. 42.4 - What is metabolic engineering? Besides...Ch. 42.4 - What is high-throughput screening and why has it...Ch. 42.4 - Prob. 5CCCh. 42.5 - Prob. 1MICh. 42.5 - What is the Ti plasmid and how is it modified for...Ch. 42.5 - How is Bt toxin produced and why is it so widely...Ch. 42.5 - Prob. 3CCCh. 42.6 - Why are diatoms and magnetotactic bacteria of...Ch. 42.6 - Prob. 2CCCh. 42 - Prob. 1RCCh. 42 - Prob. 2RCCh. 42 - Prob. 3RCCh. 42 - Prob. 4RCCh. 42 - Prob. 5RCCh. 42 - Prob. 6RCCh. 42 - Prob. 7RCCh. 42 - Prob. 1ALCh. 42 - Prob. 2AL
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- Why is Agrobacterium mediated genetic transformation described as Natural Genetic engineering in plants?arrow_forwardExplain the THREE (3) important regions of this plasmid that enable it to work efficiently as a cloning vector?arrow_forwardIRRI is currently in the process of developing a new variety of transgenic rice called Golden Rice. What is a transgenic organism? Describe what makes Golden Rice a transgenic organism. Why was Golden Rice developed?arrow_forward
- a) How are mutations beneficial for plant breeding? Discuss with an example b) Explain in brief the technology that made us self-sufficient in food production.arrow_forwardAgrobacterium-mediated plant transformation is a method used to create transgenic plants. Agrobacterium tumefaciens, a soil bacterium works exceptionally well as a natural genetic engineer. Describe briefly the creation of a transgenic plant using A. tumefaciens with illustration.arrow_forwardWhy is “Agrobacterium-mediated genetic transformation” in plants described as natural genetic engineer of plants?arrow_forward
- What is the difference between a transgenic plant and a plant produced through selective breeding?arrow_forward2a) With the aid of diagrams, describe the critical role of restriction enzymes in genetic engineering? b) Write short notes on any two characteristics of a vectorarrow_forwardJackson Wang is a biologist working with the genetics of a thermophilic bacterium. He cloned a heat shock gene from the bacteria for further analysis. After cloning, he isolated the plasmid carrying his gene of interest for sequencing. Jackson finally received the nucleotide sequence of his gene. Explain in detail how he could verify whether the nucleotide sequence matches his gene of interest using the bioinformatics databases available.arrow_forward
- A) Outline the experimental procedure for cloning a eukaryotic gene and expressing it in E. coli. Focus on the essential steps starting with eukaryotic gene amplification to transformation of E. coli cells B) Explain how insertional inactivation can help you identify the colonies that carry the plasmid with your eukaryotic gene of interest C) Plasmids containing antibiotic resistance genes are widely used in gene cloning and other molecular biology techniques. What would happen if the eukaryotic gene was inserted into an antibiotic resistance gene on the plasmid?arrow_forwardHow can site-directed mutagenesis be useful to enzymologists?arrow_forwardWhat would be an advantage of using HaeIII for a cloning experiment? What would be a disadvantage?arrow_forward
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