Jackson Wang is a biologist working with the genetics of a thermophilic bacterium. He cloned a heat shock gene from the bacteria for further analysis. After cloning, he isolated the plasmid carrying his gene of interest for sequencing. Jackson finally received the nucleotide sequence of his gene. Explain in detail how he could verify whether the nucleotide sequence matches his gene of interest using the bioinformatics databases available.
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
![Jackson Wang is a biologist working with the genetics of a thermophilic bacterium. He cloned
a heat shock gene from the bacteria for further analysis. After cloning, he isolated the plasmid
carrying his gene of interest for sequencing. Jackson finally received the nucleotide sequence
of his gene. Explain in detail how he could verify whether the nucleotide sequence matches his
gene of interest using the bioinformatics databases available.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F4bb204b4-a95f-406f-96a1-7a0fec101ddc%2F64789707-9747-44a0-a5e3-6fc5a8cf59c3%2Fi9xunb9_processed.png&w=3840&q=75)
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- Gene cloning is a frequent process in molecular biology labs where researchers make clones of a specific gene for downstream applications such as mutagenesis, genotyping, sequencing, or heterologous protein production.
- The classic gene cloning method involves transferring a gene of interest from one organism to a self-replicating genetic material, such as a bacterial plasmid.
- Cloning any DNA fragment includes seven stages in conventional molecular cloning experiments: (1) Choosing a host organism and cloning vector; (2) preparing the cloning vector's DNA; (3) preparing the DNA to be cloned; (4) producing recombinant DNA; (5) inserting recombinant DNA into the host organism; (6) choosing the organisms that contain recombinant DNA; and (7) looking for clones with the desired DNA inserts and biological characteristics.
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