Campbell Biology in Focus, Books a la Carte Edition; Modified Mastering Biology with Pearson eText - ValuePack Access Card - for Campbell Biology in Focus (2nd Edition)
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Chapter 4, Problem 9TYU

SCIENTIFIC INQUIRY In studying micrographs of an unusual protist (single-celled eukaryote) that you found in a sample of pond water, you spot an organelle that you can’t recognize. You successfully develop a method for growing this organism in liquid in the laboratory. Describe how you would go about finding out what this organelle is and what it does in the cell. Assume that you would make use of additional microscopy, cell fractionation, and biochemical tests.

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The Size of Cells and Their Components: a. (a) If you were to magnify a cell 10,000 fold (typical of the magnification achieved using an electron microscope), how big would it appear? Assume you are viewing a "typical" eukaryotic cell with a cellular diameter of 50 um.
You are studying amoebas and paramecia, two different single-celled protozoans. You have at your disposal a standard white light (known as "bright-field") microscope, a fluorescence microscope, a scanning electron microscope, and a transmission electron microscope. For each of the following studies, indicate which type of microscope would be most useful. Items (6 items) (Drag and drop into the appropriate area below) Take movies of unmanipulated cells swimming or crawling in a drop of water. Categories View three- dimensional images of the outer surface of a paramecium, including cilia. bright-field Drag and drop here View cytoplasmic streaming (movement of organelles within the cytoplasm) in a paramecium. fluorescence Drag and drop here Stain and visualize chromosomes and mitotic spindles in dividing amoebae. Visualize ribosomes in the cytosol and mitochondria of amoebas. scanning electron Drag and drop here Use labeled antibodies to locate and make color images of lysosomes in both…
Scanning electron microscope can be used for () A. Observe images of different sections of cells B. Observe living cells C. Quantitative analysis of chemical composition in cells D. Observation of cell surface stereomorphology E. Observation of cellular activity

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Campbell Biology in Focus, Books a la Carte Edition; Modified Mastering Biology with Pearson eText - ValuePack Access Card - for Campbell Biology in Focus (2nd Edition)

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