Campbell Biology in Focus, Books a la Carte Edition; Modified Mastering Biology with Pearson eText - ValuePack Access Card - for Campbell Biology in Focus (2nd Edition)
2nd Edition
ISBN: 9780134433769
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman
Publisher: PEARSON
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Question
Chapter 4.1, Problem 2CC
(a)
Summary Introduction
To determine:
The type of the microscope that you use to study (a) the change in shape of a living white blood cell and (b) the details of surface texture of a hair.
Introduction:
Microscope is an instrument that is used to see objects that are not visible from the naked eyes. It was first used by scientist of Renaissance. There are three important parameters in microscopy which are magnification, resolution and contrast.
To determine: The type of the microscope that you use to study the change in shape of a living white blood cell.
(b)
Summary Introduction
To determine: The type of the microscope that you use to study the details of surface texture of a hair.
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Students have asked these similar questions
1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 4 Solutions
Campbell Biology in Focus, Books a la Carte Edition; Modified Mastering Biology with Pearson eText - ValuePack Access Card - for Campbell Biology in Focus (2nd Edition)
Ch. 4.1 - Prob. 1CCCh. 4.1 - Prob. 2CCCh. 4.2 - Briefly describe the structure and function of the...Ch. 4.2 - Prob. 2CCCh. 4.3 - What role do ribosomes play in carrying out...Ch. 4.3 - Describe the molecular composition of nucleoli,...Ch. 4.3 - WHAT IF? As a cell begins the process of dividing,...Ch. 4.4 - Describe the structural and functional...Ch. 4.4 - Describe how transport vesicles integrate the...Ch. 4.4 - WHAT IF? Imagine a protein that functions in the...
Ch. 4.5 - Describe two characteristics shared by...Ch. 4.5 - Prob. 2CCCh. 4.5 - Prob. 3CCCh. 4.6 - Prob. 1CCCh. 4.6 - WHAT IF? Males afflicted with Kartageners syndrome...Ch. 4.7 - In what way are the cells of plants and animals...Ch. 4.7 - Prob. 2CCCh. 4.7 - MAKE CONNECTIONS The polypeptide chain that makes...Ch. 4 - Which structure is not part of the endomembrane...Ch. 4 - Which structure is common to plant and animal...Ch. 4 - Which of the following is present in a prokaryotic...Ch. 4 - Prob. 4TYUCh. 4 - Cyanide binds to at least one molecule involved in...Ch. 4 - What is the most likely pathway taken by a newly...Ch. 4 - Which cell would be best for studying lysosomes?...Ch. 4 - DRAW IT From memory, draw two eukaryotic cells....Ch. 4 - SCIENTIFIC INQUIRY In studying micrographs of an...Ch. 4 - FOCUS ON EVOLUTION Compare different aspects of...Ch. 4 - FOCUS ON ORGANIZATION Considering some of the...Ch. 4 - Prob. 12TYU
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