Oxygen consumed by a living organism in aerobic reactions is used in adding mass to the organism and/or the production of chemicals and carbon dioxide. Since we may not know the molecular compositions of all species in such a reaction, it is common to define the ratio of moles of CO2produced per mole of O2 consumed as the respiratory quotient. RQ. where
Since it generally is impossible to predict values of RQ, they must be determined from operating data. Mammalian cells are used in a bioreactor to convert glucose to glutamic acid by the reaction
CftHiiOf, + £/NHj + hO2 —? PC5H9NO4 + t/CO2 + rfl2O
The feed to the bioreactor comprises l.OOx 102 mol C6H12O(/day, 1.20x 102 mol NHj/day, and l.lOx 102 mol O2/day. Data on the system show that RQ = 0.45 mol CO2 produced/mol On consumed.
- Determine the five stoichiometric coefficients and the limiting reactant.
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