The value of research on genes that controls flowering to the society. Concept introduction: Flower is a reproductive part of a modified thick shoot of an angiosperm plant. The flowering plants generate offspring through a process called reproduction. The reproduction in plants can be sexual or asexual . Sexual reproduction is a bi-parental process (both male and female gametes are involved), whereas asexual reproduction is a uni-parental process (only one parent plant is involved).

Question

1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results? 2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab? 3) Why are the cells incubated at 42°C?

Answer 1

Question

Chapter 37, Problem 17TYU

Summary Introduction

To determine: The value of research on genes that controls flowering to the society.

Concept introduction: Flower is a reproductive part of a modified thick shoot of an angiosperm plant. The flowering plants generate offspring through a process called reproduction. The reproduction in plants can be sexual or asexual. Sexual reproduction is a bi-parental process (both male and female gametes are involved), whereas asexual reproduction is a uni-parental process (only one parent plant is involved).

Summary Introduction

To determine: The value of the research on genes that controls seed germination to the society.

Concept introduction: Flower is a reproductive part of a modified thick shoot of an angiosperm plant. The flowering plants generate offspring through a process called reproduction. The reproduction in plants can be sexual or asexual. Sexual reproduction is a bi-parental process (both male and female gametes are involved), whereas asexual reproduction is a uni-parental process (only one parent plant is involved).

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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results? 2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab? 3) Why are the cells incubated at 42°C?

Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…

Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…