Mindtap Biology, 1 Term (6 Months) Printed Access Card For Solomon/martin/martin/berg's Biology, 11th
11th Edition
ISBN: 9781337393096
Author: Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg
Publisher: Cengage Learning
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Chapter 37, Problem 6TYU
The nutritive tissue in the seeds of flowering plants that is formed from the union of a sperm cell and two polar nuclei is called the (a) plumule (b) endosperm (c) cotyledon (d) hypocotyl (e) radical
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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 37 Solutions
Mindtap Biology, 1 Term (6 Months) Printed Access Card For Solomon/martin/martin/berg's Biology, 11th
Ch. 37.1 - Prob. 1LOCh. 37.1 - Prob. 2LOCh. 37.1 - Prob. 1CCh. 37.1 - Prob. 2CCh. 37.1 - Prob. 3CCh. 37.1 - Prob. 4CCh. 37.2 - Compare the evolutionary adaptations that...Ch. 37.2 - Prob. 4LOCh. 37.2 - Prob. 1CCh. 37.2 - Prob. 2C
Ch. 37.2 - Give three examples in which the relationship of a...Ch. 37.3 - Prob. 5LOCh. 37.3 - Prob. 6LOCh. 37.3 - Explain the relationships among ovules, ovaries,...Ch. 37.3 - Distinguish among simple, aggregate, multiple, and...Ch. 37.3 - Prob. 1CCh. 37.3 - What are the main stages of eudicot embryonic...Ch. 37.3 - Prob. 3CCh. 37.3 - Prob. 4CCh. 37.3 - What are some characteristics of animal-dispersed...Ch. 37.4 - Prob. 9LOCh. 37.4 - Prob. 1CCh. 37.4 - How does early seedling development differ in...Ch. 37.5 - Explain how the following structures may be used...Ch. 37.5 - Prob. 11LOCh. 37.5 - How are rhizomes and tubers involved in asexual...Ch. 37.5 - Prob. 2CCh. 37.5 - Prob. 3CCh. 37.6 - Prob. 12LOCh. 37.6 - Prob. 1CCh. 37.6 - Prob. 2CCh. 37 - The normal order of whorls from the flowers...Ch. 37 - Prob. 2TYUCh. 37 - Prob. 3TYUCh. 37 - Prob. 4TYUCh. 37 - The process of _______________ in flowering plants...Ch. 37 - The nutritive tissue in the seeds of flowering...Ch. 37 - Prob. 7TYUCh. 37 - In plants that lack endosperm in their mature...Ch. 37 - Prob. 9TYUCh. 37 - A horizontal underground stem that may or may not...Ch. 37 - Place the following events in the correct order:...Ch. 37 - Prob. 12TYUCh. 37 - Prob. 13TYUCh. 37 - Prob. 14TYUCh. 37 - Which type of reproduction, sexual or asexual,...Ch. 37 - Prob. 16TYUCh. 37 - Prob. 17TYU
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