CAMPBELL BIOLOGY-MASTERING BIO.ACCESS
12th Edition
ISBN: 9780136486787
Author: Urry
Publisher: SAVVAS L
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Chapter 36.2, Problem 4CC
Summary Introduction
To explain: What happens if plant protoplasts are kept in pure water?
Concept introduction: Protoplasts are isolated plant cells which lack the rigid cell wall that is completely or partially removed using either mechanical or enzymatic methods. The cell wall surrounds plasma membrane and protects the cell from bursting.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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Chapter 36 Solutions
CAMPBELL BIOLOGY-MASTERING BIO.ACCESS
Ch. 36.1 - Why is long-distance transport important for...Ch. 36.1 - Some plants can detect increased levels of light...Ch. 36.1 - WHAT IF? If you prune a plant's shoot tips, what...Ch. 36.2 - If a plant cell immersed in distilled water has a...Ch. 36.2 - Prob. 2CCCh. 36.2 - How would the long-distance transport of water be...Ch. 36.2 - Prob. 4CCCh. 36.3 - Prob. 1CCCh. 36.3 - WHAT IF? Suppose an Arabidopsis mutant lacking...Ch. 36.3 - MAKE CONNECTIONS How are the Casparian strip and...
Ch. 36.4 - Prob. 1CCCh. 36.4 - Prob. 2CCCh. 36.4 - WHAT IF? If you buy cut flowers, why might the...Ch. 36.4 - Prob. 4CCCh. 36.5 - Prob. 1CCCh. 36.5 - Prob. 2CCCh. 36.5 - Prob. 3CCCh. 36.5 - WHAT IF? Apple growers in Japan sometimes make a...Ch. 36.6 - How do plasmodesmata differ from gap junctions?Ch. 36.6 - Prob. 2CCCh. 36.6 - WHAT IF? Suppose plants were genetically modified...Ch. 36 - How did the evolution of xylem and phloem...Ch. 36 - Is xylem sap usually pulled or pushed up the...Ch. 36 - Prob. 36.3CRCh. 36 - Prob. 36.4CRCh. 36 - Prob. 36.5CRCh. 36 - By what mechanisms is symplastic communication...Ch. 36 - Prob. 1TYUCh. 36 - Prob. 2TYUCh. 36 - Prob. 3TYUCh. 36 - Photosynthesis ceases when leaves wilt, mainly...Ch. 36 - Prob. 5TYUCh. 36 - A plant cell with a of -0.65 MPa maintains a...Ch. 36 - Compared with a cell with few aquaporin proteins...Ch. 36 - Prob. 8TYUCh. 36 - EVOLUTION CONNECTION Large brown algae called...Ch. 36 - SCIENTIFIC INQUIRY INTERPRET THE DATA A Minnesota...Ch. 36 - SCIENTIFIC INQUIRY Cotton plants wilt within a few...Ch. 36 - WRITE ABOUT A THEME: ORGANIZATION Natural...Ch. 36 - Prob. 13TYU
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