Biology: The Unity and Diversity of Life (MindTap Course List)
15th Edition
ISBN: 9781337408332
Author: Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr
Publisher: Cengage Learning
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Chapter 36, Problem 3DAA
Risks of Hypertension Eleni Rapsomaniki and her colleagues analyzed medical records of 1.25 million people in the United Kingdom to see how hypertension affects the risk of cardiovascular disorders. They looked at records of people who at 30 years old had no history of heart disease or stroke, although some had high blood pressure. By looking at which of these people later had a heart attack or stroke, the researchers were able to estimate the lifetime risks of these disorders in people with, and without high blood pressure. FIGURE 36.19 shows the researchers’ estimates of the lifetime risk for heart attack and for transient ischemic attack (TIA), a type of stroke.
FIGURE 36.19 Lifetime risks of heart attack and TIA (a type of stroke), with and without hypertension at age 30. A transient ischaemic attack (TIA), sometimes called a mini-stroke, is caused by a temporary blockage of a blood vessel that supplies blood to the brain.
Which is a person who has hypertension at age 30 more likely to have by age 60, a heart attack or a TIA?
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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Chapter 36 Solutions
Biology: The Unity and Diversity of Life (MindTap Course List)
Ch. 36 - Prob. 1DAACh. 36 - Prob. 2DAACh. 36 - Risks of Hypertension Eleni Rapsomaniki and her...Ch. 36 - Prob. 1SQCh. 36 - Prob. 2SQCh. 36 - Prob. 3SQCh. 36 - Prob. 4SQCh. 36 - Prob. 5SQCh. 36 - Prob. 6SQCh. 36 - Blood flows directly from the left atrium to...
Ch. 36 - Contraction of the _________ drives blood out into...Ch. 36 - Blood pressure is highest in the _______ and...Ch. 36 - In a person at rest, the largest volume of blood...Ch. 36 - Which of the following has the thickest wall? a....Ch. 36 - Prob. 12SQCh. 36 - Which artery carries oxygen-poor blood?Ch. 36 - Prob. 14SQCh. 36 - Match the terms with their definition. _____ bone...Ch. 36 - Suppose a blood clot forms in the leg, then breaks...Ch. 36 - An atrial septal defect is a birth defect in which...Ch. 36 - Prob. 3CTCh. 36 - Prob. 4CT
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