Biology: The Unity and Diversity of Life (MindTap Course List)
15th Edition
ISBN: 9781337408332
Author: Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr
Publisher: Cengage Learning
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Chapter 36, Problem 3CT
Summary Introduction
To explain: Why a low concentration of blood albumin can cause edema.
Concept introduction: Liver is one of the cardinal organs in the human body. It is mainly involved in fat
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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Chapter 36 Solutions
Biology: The Unity and Diversity of Life (MindTap Course List)
Ch. 36 - Prob. 1DAACh. 36 - Prob. 2DAACh. 36 - Risks of Hypertension Eleni Rapsomaniki and her...Ch. 36 - Prob. 1SQCh. 36 - Prob. 2SQCh. 36 - Prob. 3SQCh. 36 - Prob. 4SQCh. 36 - Prob. 5SQCh. 36 - Prob. 6SQCh. 36 - Blood flows directly from the left atrium to...
Ch. 36 - Contraction of the _________ drives blood out into...Ch. 36 - Blood pressure is highest in the _______ and...Ch. 36 - In a person at rest, the largest volume of blood...Ch. 36 - Which of the following has the thickest wall? a....Ch. 36 - Prob. 12SQCh. 36 - Which artery carries oxygen-poor blood?Ch. 36 - Prob. 14SQCh. 36 - Match the terms with their definition. _____ bone...Ch. 36 - Suppose a blood clot forms in the leg, then breaks...Ch. 36 - An atrial septal defect is a birth defect in which...Ch. 36 - Prob. 3CTCh. 36 - Prob. 4CT
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