HUMAN A+P MOD.MASTERING
2nd Edition
ISBN: 9780136919520
Author: AMERMAN
Publisher: PEARSON
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Chapter 3.3, Problem 9QC
Explain the basic process of exocytosis.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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Chapter 3 Solutions
HUMAN A+P MOD.MASTERING
Ch. 3.1 - What general processes are carried out by cells?Ch. 3.1 - Prob. 2QCCh. 3.1 - Where are intracellular and extracellular fluids...Ch. 3.2 - 1. How do phospholipids arrange themselves in the...Ch. 3.2 - 2. How is the plasma membrane described according...Ch. 3.2 - 3. What are five functions of membrane proteins?
Ch. 3.2 - What roles do cholesterol, glycoproteins, and...Ch. 3.3 - Mark each of the following statements as true or...Ch. 3.3 - Mark each of the following statements as true or...Ch. 3.3 - Mark each of the following statements as true or...
Ch. 3.3 - Mark each of the following statements as true or...Ch. 3.3 - How does the process of primary active transport...Ch. 3.3 - What is the main primary active transport pump in...Ch. 3.3 - 7. How does the process of secondary active...Ch. 3.3 - Prob. 8QCCh. 3.3 - Explain the basic process of exocytosis.Ch. 3.4 - 1. Identify the properties listed in the next...Ch. 3.4 - Identify the following properties as belonging to...Ch. 3.4 - To what destinations can products from the Golgi...Ch. 3.5 - Prob. 1QCCh. 3.5 - Prob. 2QCCh. 3.5 - Prob. 3QCCh. 3.6 - 1. What are the main components of the nucleus?...Ch. 3.6 - What is chromatin? How are chromatin and...Ch. 3.6 - 3. What is a nucleolus, and what is its...Ch. 3.7 - How is a codon related to a triplet?Ch. 3.7 - 2. Describe the basic steps of transcription.
Ch. 3.7 - Explain how tRNA acts as the translator of the...Ch. 3.7 - Describe the basic steps of translation.Ch. 3.7 - 5. Why is posttranslational modification...Ch. 3.7 - 6. Why is it important to regulate gene...Ch. 3.8 - What happens during each stage of the cell cycle?Ch. 3.8 - What does semiconservative replication mean?Ch. 3.8 - Describe the changes in the cell that take place...Ch. 3.8 - What are four external factors that play a role in...Ch. 3 - Which of the following is not a basic function...Ch. 3 - Prob. 2CYRCh. 3 - What are the two fluid compartments in the body,...Ch. 3 - 4. Which of the following best describes the...Ch. 3 - Mark the following statements about the plasma...Ch. 3 - 6. What is the primary difference between active...Ch. 3 - 7. Match the term with its appropriate...Ch. 3 - 8. Fill in the blanks: A hypotonic solution will...Ch. 3 - 9. Match the following terms with the correct...Ch. 3 - Prob. 10CYRCh. 3 - Mark the following statements about the...Ch. 3 - 12. Our somatic cells’ DNA is distributed among...Ch. 3 - Explain how and why chromatin is condensed in the...Ch. 3 - Which of the following statements correctly...Ch. 3 - Each of the following statements about protein...Ch. 3 - Number the following steps of protein synthesis in...Ch. 3 - Which of the following is not a phase of mitosis?...Ch. 3 - 18. Why is regulation of the cell cycle...Ch. 3 - 19. Mark the following statements about the cell...Ch. 3 - 20. Match the following terms with the correct...Ch. 3 - 1. Write a single sentence, using no more than 25...Ch. 3 - 2. Certain diseases are transmitted via...Ch. 3 - 3. Explain how the form of each of the following...Ch. 3 - Certain types of cancerous lung tumors can secrete...Ch. 3 - Why do you think the rate of cell division is...Ch. 3 - 1. A patient is admitted to the hospital and...Ch. 3 - A popular science fiction program once had an...Ch. 3 - Prob. 3AYKACh. 3 - Prob. 4AYKACh. 3 - The drug methotrexate is used to treat several...
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- You will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. You have already cultured it and gone through the plate isolation procedure. Before you ship your samples off for sequencing, you want to do one final check of the A260 ratios. You get back the following ratios: A260/280 ratio is 1.89; A260/230 is 2.01. These ratios are close enough to the accepted "pure" values so they could be considered "pure" and mostly (if not completely) free of contaminants from the PCR process. True Falsearrow_forwardYou will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. After receiving your sequence back from the sequencing lab, you feel that you have, in fact, discovered and isolated a new species. You ask a fellow labmate about how you should proceed, and he tells you the following is the proper way to introduce a new species for recognition: Cultures have to be sent to international culture collections. Then a paper must be published describing the new organism and providing a genus and species name. You recall learning about this in your Microbiology course in college. Is this information from your colleague true or false? True Falsearrow_forwardis often a good indication of phylogenetic relatedness in phenotypes. Life-cycle patterns Cleavage patterns O Gene expression O Morphological similarityarrow_forward
- Which of the following is a weakness of using 16S rRNA for phylogenetic analyses? It can only go down to the family and genus levels It takes months to complete O Both of the above O None of the abovearrow_forwardAn unrooted tree containing ten unrelated species can become rooted by adding a descendant group related to two of the species. an unrelated outgroup. O a distantly related outgroup. O a descendant related to only one of the species.arrow_forwardWhat is the most appropriate purpose of building a phylogenetic tree? They look awesome You can use a tree to compare morphological characteristics of organisms It can be used to establish and analyze evolutionary relationships between species All of the abovearrow_forward
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