Brock Biology of Microorganisms (14th Edition)
14th Edition
ISBN: 9780321897398
Author: Michael T. Madigan, John M. Martinko, Kelly S. Bender, Daniel H. Buckley, David A. Stahl, Thomas Brock
Publisher: PEARSON
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Textbook Question
Chapter 3.2, Problem 2MQ
In which medium shown in Table 5.1, defined or complex, do you think Escherichia coli would grow the fastest? Why? E. coli will not grow in the medium described for Thiobacillus thioparus. Why?
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Chapter 3 Solutions
Brock Biology of Microorganisms (14th Edition)
Ch. 3.1 - Which four chemical elements make up the bulk of a...Ch. 3.1 - Which two classes of macromolecules contain most...Ch. 3.1 - Prob. 3MQCh. 3.2 - Why would a complex culture medium for Leuconostoc...Ch. 3.2 - In which medium shown in Table 5.1, defined or...Ch. 3.2 - What is meant by the word sterile? Why is aseptic...Ch. 3.3 - Prob. 1MQCh. 3.3 - Prob. 2MQCh. 3.4 - What is free energy?Ch. 3.4 - Prob. 2MQ
Ch. 3.4 - Using Table 3.2, calculate G0 for the reaction...Ch. 3.5 - Prob. 1MQCh. 3.5 - Prob. 2MQCh. 3.5 - Prob. 3MQCh. 3.6 - Prob. 1MQCh. 3.6 - Prob. 2MQCh. 3.6 - Prob. 3MQCh. 3.7 - How much free energy is released when ATP is...Ch. 3.7 - Prob. 2MQCh. 3.7 - Prob. 3MQCh. 3.8 - Which reactions in glycolysis are redox steps?Ch. 3.8 - Prob. 2MQCh. 3.8 - Prob. 3MQCh. 3.9 - Prob. 1MQCh. 3.9 - Prob. 2MQCh. 3.10 - Prob. 1MQCh. 3.10 - Which electron carriers described in this section...Ch. 3.11 - How do electron transport reactions generate the...Ch. 3.11 - Prob. 2MQCh. 3.11 - What structure in the cell links the proton motive...Ch. 3.12 - Prob. 1MQCh. 3.12 - What two major roles do the citric acid cycle and...Ch. 3.12 - Why is the glyoxylate cycle necessary for growth...Ch. 3.13 - Prob. 1MQCh. 3.13 - Prob. 2MQCh. 3.13 - Prob. 3MQCh. 3.14 - What form of activated glucose is used in the...Ch. 3.14 - Prob. 2MQCh. 3.14 - What functions does the pentose phosphate pathway...Ch. 3.15 - Prob. 1MQCh. 3.15 - List the steps required for the cell to...Ch. 3.15 - Which nitrogen bases are purines and which are...Ch. 3.16 - Prob. 1MQCh. 3.16 - Prob. 2MQCh. 3.17 - Prob. 1MQCh. 3.17 - Prob. 2MQCh. 3.17 - Prob. 3MQCh. 3 - Prob. 1RQCh. 3 - Prob. 2RQCh. 3 - What is aseptic technique and why is it necessary?...Ch. 3 - Prob. 4RQCh. 3 - REVIEW QUESTIONS
5. Describe how you would...Ch. 3 - Prob. 6RQCh. 3 - Why are enzymes needed by the cell? (Section 3.5)
Ch. 3 - Prob. 8RQCh. 3 - 9. What is the reduction potential of the...Ch. 3 - Prob. 10RQCh. 3 - How is ATP made in fermentation and in...Ch. 3 - Prob. 12RQCh. 3 - MINIQUIZ
13. Besides lactic acid and ethanol, list...Ch. 3 - List some of the key electron carriers found in...Ch. 3 - What is meant by proton motive force and how is it...Ch. 3 - Prob. 16RQCh. 3 - MINIQUIZ
17. How much more ATP is possible in...Ch. 3 - Prob. 18RQCh. 3 - MINIQUIZ
19. What is the major difference between...Ch. 3 - Prob. 20RQCh. 3 - Describe the process by which a fatty acid such as...Ch. 3 - Prob. 22RQCh. 3 - Prob. 1AQCh. 3 - Application Questions
2. Desulfovibrio can grow...Ch. 3 - Using the data of Figure 3.10, predict the...Ch. 3 - Prob. 4AQ
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- Sydney Brenner isolated Salmonella typhimurium mutants that were implicated in the biosynthesis of tryptophan and would not grow on minimal medium. When these bacterial mutants were tested on minimal medium to which one of four compounds (indole glycerol phosphate, indole, anthranilic acid, and tryptophan) had been added, the growth responses shown in the following table were obtained. Mutant Minimal medium Anthranilic Indole glycerol acid Indole Tryptoph phosphate trp-1 trp-2 trp-3 trp-4 trp-6 trp-7 trp-8 trp-9 trp-10 trp-11 - Give the order of indole glycerol phosphate, indole, anthranilic acid, and tryptophan in a biochemical pathway leading to the synthesis of tryptophan. Indicate which step in the pathway is affected by each of the mutations. + 1 + 1 IIarrow_forwardSydney Brenner isolated Salmonella typhimurium mutants that were implicated in the biosynthesis of tryptophan and would not grow on minimal medium. When these bacterial mutants were tested on minimal medium to which one of four compounds (indole glycerol phosphate, indole, anthranilic acid, and tryptophan) had been added, the growth responses shown in the following table were obtained. Mutant Minimal medium Anthranilic acid Indole glycerol phosphate Indole Tryptophan trp-1 − − − − + trp-2 − − + + + trp-3 − − − + + trp-4 − − + + + trp-6 − − − − + trp-7 − − − − + trp-8 − + − − + trp-9 − − − − + trp-10 − − − − + trp-11 − − − − + Give the order of indole glycerol phosphate, indole, anthranilic acid, and tryptophan in a biochemical pathway leading to the synthesis of tryptophan. Indicate which step in the pathway is affected by each of the mutations.arrow_forwardBelow is shown a growth curve for an E. coli culture. As indicated, the culture was incubated in the absence of a carbon source for 2 hours until Glucose and Lactose were added; Glucose was used up after 5 hours, and Lactose was used up after 8 hours. During the time-course, you take four samples, labeled A-D, after 1, 3, 7 and 9 hours, respectively, as indicated under the graph. Assuming there is cAMP present at t=0, for each sample identify whether CAP and/or the Lac Repressor would be bound to the DNA, and explain why.arrow_forward
- Pseudomonas bacteria have porin proteins, are resistant to the chemical triclosan, and survive and can even multiply in quaternary ammonium compounds. True or False?arrow_forwardExplain why the growth of both the strains A and C in medium III is not similar to that of strain B. Use the appropriate vocabulary and provide one putative explanation for strain A.arrow_forwardMenaquinones is produced by Flavobacterium meningosepticum via submerged fermentation with 60% working volume in 30-L fermentation. To obtain the crude menaquinones, the fermentation medium is centrifuged at 12,000 rpm for 15 minutes. The vacuum-freeze dried particles are crushed and collected. After that the samples are treated with methanol for 20 minutes. To remove polar lipid compounds from bacterial cells, HZ816 macroporous adsorption resin column is used with methanol-dichloromethane (l:1) as mobile phase and flow rate of 0.75 mL/min. Rotary evaporation at 500 C is carried out to concentrate the eluent. After that, the concentrated sample is purified with Bio-BeadsTM S-X3 to remove components without UV absorption. To obtain the homolog purified menaquinones, RP-C18 column is used. Recrystallization is carried out to obtain pure crystalized menaquinones. Answer the following: Indicate how to obtain the sample in the methanol solvent. Three different chromatography are used in…arrow_forward
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