Brock Biology of Microorganisms (14th Edition)
14th Edition
ISBN: 9780321897398
Author: Michael T. Madigan, John M. Martinko, Kelly S. Bender, Daniel H. Buckley, David A. Stahl, Thomas Brock
Publisher: PEARSON
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Textbook Question
Chapter 3, Problem 3AQ
Using the data of Figure 3.10, predict the sequence of electron carriers in the membrane of an organism growing aerobically that has the following electron carriers: ubiquinone, cytochrome aa3, cytochrome b, NADH, cytochrome c, FAD.
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A given enzyme is able to utilize five different substrates. The Km's have
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Substrate 1 with KM= 4.7 × 10-1 M.
Substrate 2 with Km = 1.5 × 10-2 M.
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Substrate 4 with Km= 4.7 × 10-³ M
Substrate 5 with KM = 4. 7 × 10-5 M
Photosynthesis and aerobic cellular respiration both rely on electron transport chains to generate ATP. Which of the following does not correctly identify similarities and differences in the ETCs of these processes?
a) Electrons delivered to the ETC are used to generate a proton gradient across the membrane
b) In photosynthesis, the facilitated diffusion of protons across the membrane generates ATP and glucose molecules; in cellular respiration, this process generates ATP
c) In photosynthesis, electrons are delivered to the ETC by NADPH; in cellular respiration, electrons are delivered to the ETC by NADH and FADH2
d) In prokaryotes, active transport moves protons across the cell’s plasma membrane during photosynthesis and cellular respiration
The electrons that reduce nitrate to nitrite are brought to it by a short electron transport chain involving FADH2 and NADH (see Figure). In which other reactions have you seen these two electron carriers?
Chapter 3 Solutions
Brock Biology of Microorganisms (14th Edition)
Ch. 3.1 - Which four chemical elements make up the bulk of a...Ch. 3.1 - Which two classes of macromolecules contain most...Ch. 3.1 - Prob. 3MQCh. 3.2 - Why would a complex culture medium for Leuconostoc...Ch. 3.2 - In which medium shown in Table 5.1, defined or...Ch. 3.2 - What is meant by the word sterile? Why is aseptic...Ch. 3.3 - Prob. 1MQCh. 3.3 - Prob. 2MQCh. 3.4 - What is free energy?Ch. 3.4 - Prob. 2MQ
Ch. 3.4 - Using Table 3.2, calculate G0 for the reaction...Ch. 3.5 - Prob. 1MQCh. 3.5 - Prob. 2MQCh. 3.5 - Prob. 3MQCh. 3.6 - Prob. 1MQCh. 3.6 - Prob. 2MQCh. 3.6 - Prob. 3MQCh. 3.7 - How much free energy is released when ATP is...Ch. 3.7 - Prob. 2MQCh. 3.7 - Prob. 3MQCh. 3.8 - Which reactions in glycolysis are redox steps?Ch. 3.8 - Prob. 2MQCh. 3.8 - Prob. 3MQCh. 3.9 - Prob. 1MQCh. 3.9 - Prob. 2MQCh. 3.10 - Prob. 1MQCh. 3.10 - Which electron carriers described in this section...Ch. 3.11 - How do electron transport reactions generate the...Ch. 3.11 - Prob. 2MQCh. 3.11 - What structure in the cell links the proton motive...Ch. 3.12 - Prob. 1MQCh. 3.12 - What two major roles do the citric acid cycle and...Ch. 3.12 - Why is the glyoxylate cycle necessary for growth...Ch. 3.13 - Prob. 1MQCh. 3.13 - Prob. 2MQCh. 3.13 - Prob. 3MQCh. 3.14 - What form of activated glucose is used in the...Ch. 3.14 - Prob. 2MQCh. 3.14 - What functions does the pentose phosphate pathway...Ch. 3.15 - Prob. 1MQCh. 3.15 - List the steps required for the cell to...Ch. 3.15 - Which nitrogen bases are purines and which are...Ch. 3.16 - Prob. 1MQCh. 3.16 - Prob. 2MQCh. 3.17 - Prob. 1MQCh. 3.17 - Prob. 2MQCh. 3.17 - Prob. 3MQCh. 3 - Prob. 1RQCh. 3 - Prob. 2RQCh. 3 - What is aseptic technique and why is it necessary?...Ch. 3 - Prob. 4RQCh. 3 - REVIEW QUESTIONS
5. Describe how you would...Ch. 3 - Prob. 6RQCh. 3 - Why are enzymes needed by the cell? (Section 3.5)
Ch. 3 - Prob. 8RQCh. 3 - 9. What is the reduction potential of the...Ch. 3 - Prob. 10RQCh. 3 - How is ATP made in fermentation and in...Ch. 3 - Prob. 12RQCh. 3 - MINIQUIZ
13. Besides lactic acid and ethanol, list...Ch. 3 - List some of the key electron carriers found in...Ch. 3 - What is meant by proton motive force and how is it...Ch. 3 - Prob. 16RQCh. 3 - MINIQUIZ
17. How much more ATP is possible in...Ch. 3 - Prob. 18RQCh. 3 - MINIQUIZ
19. What is the major difference between...Ch. 3 - Prob. 20RQCh. 3 - Describe the process by which a fatty acid such as...Ch. 3 - Prob. 22RQCh. 3 - Prob. 1AQCh. 3 - Application Questions
2. Desulfovibrio can grow...Ch. 3 - Using the data of Figure 3.10, predict the...Ch. 3 - Prob. 4AQ
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- Indicate whether the statement is true for aerobic respiration, photosynthesis or both: The transfer of electrons via redox reactions is coupled to the movement of protons from one side of the membrane to the other a)Aerobic Respiration b)Photosynthesis c)Botharrow_forwardWhich of the following sequence of proteins and the corresponding number of pumped protons is correct during the electron transport chain from NADH? Ubiquinone → Complex III: 4 H+ → Cytochrome c→ Complex IV: 2 Ubiquinone Complex III: 2 H+→ Cytochrome c→ Complex IV:2 Ubiquinone → Cytochrome c→ Complex III: 4 H+ → Complex IV: 2 D. Complex 1: 4 H+ → Ubiquinone → Complex III: 2H+ → Complex IV: 2 H+ → Cytochrome c A. Complex I: 4 H+ → B. Complex I: 4 H+ C. Complex I: 4 H+arrow_forwardWhy are electron carriers (NAD+/NADH and FAD/FADH2) so important in the process of cellular respiration? A)NADH and FADH2 are major components of the ETC, so without them, there would be no ETC in the cell. B)They deliver electrons to the ETC, which in turn sets up chemiosmosis, where most of the ATP is generated. C)They separate the electrons from the protons so that the protons can be moved out of the mitochondrion. D)The electrons that they carry are able to directly phosphorylate ADP in order to generate the bulk of ATP in the cell. E) They transport protons across the mitochondrial membrane. 14.arrow_forward
- If the order for the electron transport chain starting from NADH to Oxygen is this: NADH-->Flavin mononucleotide (FMN)--> Coenzyme Q (CoQ) --> Cytochrome b (Cyt b)--> Cytochrome c (Cyt C)--> Cytochrome a3 (cyt a3) --> Oxygen (O2) Then, what is the order for the electron transport chain starting with FADH2 to oxygen (O2)?arrow_forwardThe diagram in Figure 5 below summarizes the electron-transport chain, which is a stage of cell respiration. Letters A. B and C represent electron carriers. 5) Hydrogen ions NADH + H* Oxidised A Reduced B Oxidised C Electrons XXXX NAD+ Reduced A Oxidised B Reduced B Figure 5 i) Explain what is happening to the electrons and hydrogen ions from carriers A to C. ii) What is happening to the electrons and hydrogens at the end of this stage? ii) State where in the mitochondrion is the electron transport chain situated? iv) Name ONE process that produces NADH + H"? How does the electron transport chain contribute to the production of ATP? v)arrow_forward5. a) The cell creates molecules of NADH and FADH2to use in the electron transport chain as they are electron carrier molecules. The electron transfers from these moleculesdrives the movement of what molecule across the mitochondrial membrane?b) What is chemiosmosis?c) How does the cell use chemiosmosis to drive overcome the thermodynamic barrier of bringing phosphates close togehter to create ATP?d) What steps of oxidative phosphorylation are in the cytosol and which are in the mitochondria?e) Cellular respiration can be controlled at the post-translational level. What does this mean, using GLUT as an example? Why is post-translational control of GLUT evolutionarilyadaptive over transcriptional control?f) How does establishment of equillibria of various molecules control metabolic reactions? What are three ways high ATP in the cell control cellular respiration?g) Why are the enzymes controlling the initial steps of various biochemical pathways are targeted by allosteric control?arrow_forward
- Complete the table ATP Yield from Anaerobic Respiration (for 1 Glucose Molecule)arrow_forwardFigure 4.15 Cyanide inhibits cytochrome c oxidase, a component of the electron transport chain. If cyanide poisoning occurs, would you expect the pH of the intermembrane space to increase or decrease? What affect would cyanide have on ATP synthesis?arrow_forwardFor each statement below, write whether the event occurs in GLYCOLYSIS, KREBS CYCLE, or ELECTRON TRANSPORT CHAIN. 1. Oxygen is the final acceptor of electrons 2. Occurs outside the mitochondria 3. Produces FADH2 4. Begins with glucose 5. Occurs in the mitochondrial matrix 6. Ends with pyruvic acid 7. Where chemiosmosis occurs 8 .Forms water 9.Occurs in the inner membrane of the mitochondria 10. Produces the most ATParrow_forward
- Table 8: ATP Yield from Anaerobic Respiration (for 1 Glucose Molecule) # of ATPS Generated through Substrate-Level Phosphorylation # of ATPS Generated from NADH and FADH, through Oxidative # of NADH and FADH, Produced Gross Subtotal of ATPS Produced Process Phosphorylation NADH: FADH2: Glycolysis NADH: FADH2: GROSS TOTAL of ATPS Produced # of ATPS Spent NETTOTAL of ATPS Produced Fermentationarrow_forwardWhich of the following statements concerning the complete oxidation of FADH2 in the electron transport chain is NOT true? a. In the final step, electrons from cytochrome c to O2 reducing it to H2O in complex IV, and four protons are transported from the intermembrane space to the matrix. b. In the first step, electrons from FADH2 are transferred in complex II to ubiquinone, which does not transport any proton across the inner mitochondrion membrane. c. In the second step, complex III transfers the electrons from ubiquinone to cytochrome c, and four protons are transported from the matrix to the intermembrane space. d. The complete oxidation of FADH2 causes transfer of 6 protons and yields two ATP.arrow_forwardIndicate whether the statement is true for aerobic respiration, photosynthesis or both: NADH is oxidized to NAD+ at complex I a)Aerobic Respiration b)Photosynthesis c)Botharrow_forward
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