The effect of pH on separation of amino acids during electrophoresis process needs to be explained. Concept introduction: Electrophoresis is a technique of separation of ions by applying electric field. In this method, the analytes can be separated through conductive medium in presence of electric field. Without this effect the cations migrate towards the negatively charged cathode and anions moves toward positively charged anode.

Question

Shown are the configuration of a gel electrophoresis experiment. Charged plates establish an electric field on a region of gel between the plates. A solution of DNA fragments, which acquire a negative charge in solution, is analyzed by using an electric field to drive the fragments through the gel. A drag force opposes the motion, causing the fragments to move at a constant speed. Larger fragments move more slowly, so the fragments separate by size to give a “fingerprint” of the sample. What is the energy transformation as the fragments move through the gel?A. Electric potential energy to kinetic energyB. Electric potential energy to thermal energyC. Electric potential energy to kinetic energy and thermal energyD. Kinetic energy to thermal energy

Answer 1

Question

Chapter 30, Problem 30.3QAP

Interpretation Introduction

Interpretation:

The effect of pH on separation of amino acids during electrophoresis process needs to be explained.

Concept introduction:

Electrophoresis is a technique of separation of ions by applying electric field. In this method, the analytes can be separated through conductive medium in presence of electric field. Without this effect the cations migrate towards the negatively charged cathode and anions moves toward positively charged anode.

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