Principles of Instrumental Analysis
7th Edition
ISBN: 9781305577213
Author: Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher: Cengage Learning
expand_more
expand_more
format_list_bulleted
Concept explainers
Question
Chapter 30, Problem 30.9QAP
Interpretation Introduction
Interpretation:
The order of elution of protein ions A2+, B+ and C3+ needs to be determined, if they are ionized at pH at which electrical FFF separation is carried out.
Concept introduction:
FFF or Field flow fractionation is a separation method in which a field is applied to solution that is pumped through channel perpendicular to the direction of flow such that the particles separate. The separation depends on the force applied by the field.
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solutionStudents have asked these similar questions
Three large proteins are ionized at the pH at which an electrical FFF separation is carried out.
(i) if the ions are designated A2+, B2+, and C3+, predict the order of elution.
(ii) What is FFF?
(iii) List the four subtechniques of FFF.
There is a class of polyesters that have the general structure shown below. For example, in polyethylene terephthalate (PET) m =1. How would you expect the Tg ad Tm of these polymers to vary with m, given that the molecular weight of the chain is held constant? Give reasons for your answer. Also, assuming that you are now just considering one member of this class of polymers, say PET, how does the Tg vary with molecular weight, n.
The volume of O2(g) adsorbed on a surface of 1 g of a silica sample at 5°C is V1=1.4 cm3 at P1=95.1 torr and V2=10.6 cm3 at P2=760 torr. Identify the maximum volume of oxygen, Vm, that can be adsorbed. Consider the Langmuir isotherm model
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.Similar questions
- Suppose the concentration of a solute decays linearly along the length of a container according to c(x) = c0 - αc0x, where c0 is the concentration at x = 0. Calculate the thermodynamic force on the solute at 25 °C and at x = 10 cm and 20 cm given that the concentration falls to 1/2c0 when x = 10 cm. Hint: Start by finding the value of α.arrow_forwardAn amino acid on the surface of a protein was labelled covalently with 1.5-I AEDANS and another was labelled covalently with FITC. The fluorescence quantum yield of 1.5-I AEDANS decreased by 10 per cent due to quenching by FITC. What is the distance between the amino acids? (Refer to Table 17G.3 for the appropriate value of R0.)arrow_forwardA student conducted an experiment to determine the concentration of benzene in an ethanol solution. Use the UV spectrum of the solution in a 2.0 cm cell. Within the spectrum, there was a peak attributed to benzene at 260 nm, with an absorption of 0.69. In a reference source, the student found that the adsorption of the benzene strip with ethanol was 2.95 x 10-3 ppm cm-. What is the molar concentration of benzene in the sample (mm of benzene = 78.11 g/mol)arrow_forward
- The biuret reaction is a general method for the determination of proteins or peptides. This method is based on the reaction of the Cu2+ with four NH groups of the peptide bonds, forming a violet complex with an absorption maximum at 540 nm. The intensity of the color is proportional to the number of peptide bonds present, and therefore to the amount of protein. This reaction is specific, the dipeptides and amino acids give a negative reaction, and few substances interfere. A quantity of 19.89 g of fish meat is extracted with 100 mL of glacial acetic acid. Subsequently, 3 mL of the previous solution with 3 mL of the biuret reagent to generate the reaction, after 30 minutes. To reduce errors due to other interfering substances and residual turbidity, the corrected absorbance method Ac is applied to 500 nm and 570 nm. In a study, a standard sample analyzed by the Kjeldahl method (total Nitrogen analysis) is obtained, resulting in 0.43% N (w / v), (Protein content [%] =% N x 6.38). 3 mL of…arrow_forwardThe following data are for the chemisorption of hydrogen on copper powder at 25°C. Confirm that they fit the Langmuir isotherm at low coverages. Then find the value of K for the adsorption equilibrium and the adsorption volume corresponding to complete coverage. \ table V 3 [la, 25, 129,253, 540],[m², 0.042, 0.163,0.221,0.32|]] сarrow_forward19arrow_forward
- Iron nanoparticles were produced by the evaporation method and their average sizes are estimated 25,50, 90 and 120 nm using the laser particle analyzer. Assuming that the resultant particles are strain freeand the peak broadening detected in the measurement with Cu-K radiation (λ=0.154 nm) arises onlyfrom variation of size of the crystallites, estimate the peak width (the value of FWHM) of the (110) plane.arrow_forwardNitrogen gas adsorbed on a surface to the extent of 1.242 cm3 g−1 at 350 kPa and 180 K, but at 240 K the same amount of adsorption was achieved only when the pressure was increased to 1.02 MPa. What is the enthalpy of adsorption of nitrogen on the surface?arrow_forward1. Calculate the Coulombic (ionic) interaction energy between atoms for the C=O ∙∙∙H-N hydrogen bond in a peptide at a distance of 1.5Å in units of kJ/mol. Assume that the partial charge on O is −0.434, the partial charge on H is +0.417 and the permittivity of the protein environment is 6. coulimbic interaction energy= 27.95 kJ/mol 2. The dipole moment of an individual peptide group is approximately 3.46 D. Assuming that the dipoles line up linearly estimate the energy of interaction (in kJ.mol) of the hydrogen bond in Q1 using a dipole-dipole model. *******please answer #2**********arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
- Principles of Instrumental AnalysisChemistryISBN:9781305577213Author:Douglas A. Skoog, F. James Holler, Stanley R. CrouchPublisher:Cengage Learning
Principles of Instrumental Analysis
Chemistry
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Cengage Learning