The order of elution of protein ions A 2+ , B+ and C 3+ needs to be determined, if they are ionized at pH at which electrical FFF separation is carried out. Concept introduction: FFF or Field flow fractionation is a separation method in which a field is applied to solution that is pumped through channel perpendicular to the direction of flow such that the particles separate. The separation depends on the force applied by the field.

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Chapter 30, Problem 30.9QAP

Interpretation Introduction

Interpretation:

The order of elution of protein ions A²⁺, B+ and C³⁺ needs to be determined, if they are ionized at pH at which electrical FFF separation is carried out.

Concept introduction:

FFF or Field flow fractionation is a separation method in which a field is applied to solution that is pumped through channel perpendicular to the direction of flow such that the particles separate. The separation depends on the force applied by the field.

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