Principles of Instrumental Analysis
7th Edition
ISBN: 9781305577213
Author: Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher: Cengage Learning
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Chapter 30, Problem 30.6QAP
Interpretation Introduction
Interpretation:
The time taken by the analyte cation to reach the detector needs to be determined, where the osmotic flow rate is given as 0.85mm/s and detector is placed 40cm from the injection end of capillary.
Concept introduction:
The instrumentation needs for capillary electrophoresis includes electric field, cathode and anode compartments which would contain the buffer solution and small vial that contains the sample, capillary tube and detector.
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A 0.0200 gram blood sample was decomposed by a microwave digestion technique followed by dilution to 100.0 mL in a volumetric flask. Aliquots of the sample solution were treated with a lead complexing reagent and water as follows: Solution 1: 10.0 ml blood sample + 20.0 mL complexing agent + 30.0 mL H20. Solution 2: 10.0 ml blood sample + 20.0 mL complexing agent + 26.0 mL H20 + 4.00 mL of 78 ppb Pb2+ standard. The resulting solutions were analyzed by UV/Vis at 375 nm. Absorbance for solution 1 = 0.155 and for solution 2 = 0.216. Calculate the concentration of lead (ppb) in the original sample.
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An anion has an electrophoretic mobility of -2.50 cm2 /kV · min on a CE system containing a 30 cm long capillary with a detector located 25.0 cm from the
point of injection. If the electroosmotic mobility of the buffer is 4.10 cm- /kV · min, and the applied voltage is 15.0 kV, what are the migration time (v) and the
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O A. 37.5
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O C. 20.0
O D. 12.2
O E. 26.1
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