(a) Interpretation: The autoradiographic pattern for strands (i) to (v) should be determined. Concept introduction: DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA. RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The nucleotides are combined together by the enzyme RNA Polymerase.

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Answer 1

Question

Chapter 30, Problem 12P

Interpretation Introduction

(a)

Interpretation:

The autoradiographic pattern for strands (i) to (v) should be determined.

Concept introduction:

DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA.

RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The nucleotides are combined together by the enzyme RNA Polymerase.

Interpretation Introduction

(b)

Interpretation:

The possible effect of rifampicin on the RNA synthesis in the given system should be determined.

Concept introduction:

DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA.

RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The nucleotides are combined together by the enzyme RNA Polymerase.

Interpretation Introduction

(c)

Interpretation:

The reason for the difference between the heparin blocks the elongation of RNA primer before the transcription but not after should be determined.

Concept introduction:

DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA.

RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The nucleotides are combined together by the enzyme RNA Polymerase.

Interpretation Introduction

(d)

Interpretation:

The length of the longest product in the absence of four ribo-nucleoside triphosphate should be determined.

Concept introduction:

DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA.

RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The nucleotides are combined together by the enzyme RNA Polymerase.

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The beta-lactamase hydrolyzes the lactam-ring in penicillin. Describe the mechanism of hydrolysis, insuring to include the involvement of S, D, & K in the reaction sequence. Please help

To map the active site of beta-lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine. Why doesn't D in this hexapeptide not participate in the hydrolysis of the beta-lactam ring even though S, K, and D are involved in the catalyst?

To map the active site of -lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine. Using the experimental results described above derive the primary sequence of the active site hexapeptide. Please help!

Answer 2

Question

Chapter 30, Problem 12P

Interpretation Introduction

(a)

Interpretation:

The autoradiographic pattern for strands (i) to (v) should be determined.

Concept introduction:

DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA.

RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The nucleotides are combined together by the enzyme RNA Polymerase.

Interpretation Introduction

(b)

Interpretation:

The possible effect of rifampicin on the RNA synthesis in the given system should be determined.

Concept introduction:

DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA.

RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The nucleotides are combined together by the enzyme RNA Polymerase.

Interpretation Introduction

(c)

Interpretation:

The reason for the difference between the heparin blocks the elongation of RNA primer before the transcription but not after should be determined.

Concept introduction:

DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA.

RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The nucleotides are combined together by the enzyme RNA Polymerase.

Interpretation Introduction

(d)

Interpretation:

The length of the longest product in the absence of four ribo-nucleoside triphosphate should be determined.

Concept introduction:

DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA.

RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The nucleotides are combined together by the enzyme RNA Polymerase.

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Answer 3

Question

Chapter 30, Problem 12P

Interpretation Introduction

(a)

Interpretation:

The autoradiographic pattern for strands (i) to (v) should be determined.

Concept introduction:

DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA.

RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The nucleotides are combined together by the enzyme RNA Polymerase.

Interpretation Introduction

(b)

Interpretation:

The possible effect of rifampicin on the RNA synthesis in the given system should be determined.

Concept introduction:

DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA.

RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The nucleotides are combined together by the enzyme RNA Polymerase.

Interpretation Introduction

(c)

Interpretation:

The reason for the difference between the heparin blocks the elongation of RNA primer before the transcription but not after should be determined.

Concept introduction:

DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA.

RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The nucleotides are combined together by the enzyme RNA Polymerase.

Interpretation Introduction

(d)

Interpretation:

The length of the longest product in the absence of four ribo-nucleoside triphosphate should be determined.

Concept introduction:

DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA.

RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The nucleotides are combined together by the enzyme RNA Polymerase.

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Answer 4

Question

Chapter 30, Problem 12P

Interpretation Introduction

(a)

Interpretation:

The autoradiographic pattern for strands (i) to (v) should be determined.

Concept introduction:

DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA.

RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The nucleotides are combined together by the enzyme RNA Polymerase.

Interpretation Introduction

(b)

Interpretation:

The possible effect of rifampicin on the RNA synthesis in the given system should be determined.

Concept introduction:

DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA.

RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The nucleotides are combined together by the enzyme RNA Polymerase.

Interpretation Introduction

(c)

Interpretation:

The reason for the difference between the heparin blocks the elongation of RNA primer before the transcription but not after should be determined.

Concept introduction:

DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA.

RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The nucleotides are combined together by the enzyme RNA Polymerase.

Interpretation Introduction

(d)

Interpretation:

The length of the longest product in the absence of four ribo-nucleoside triphosphate should be determined.

Concept introduction:

DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA.

RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The nucleotides are combined together by the enzyme RNA Polymerase.

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Answer 5

Chapter 30, Problem 12P

Interpretation Introduction

(a)

Interpretation:

The autoradiographic pattern for strands (i) to (v) should be determined.

Concept introduction:

DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA.

RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The nucleotides are combined together by the enzyme RNA Polymerase.

Interpretation Introduction

(b)

Interpretation:

The possible effect of rifampicin on the RNA synthesis in the given system should be determined.

Concept introduction:

DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA.

RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The nucleotides are combined together by the enzyme RNA Polymerase.

Interpretation Introduction

(c)

Interpretation:

The reason for the difference between the heparin blocks the elongation of RNA primer before the transcription but not after should be determined.

Concept introduction:

DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA.

RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The nucleotides are combined together by the enzyme RNA Polymerase.

Interpretation Introduction

(d)

Interpretation:

The length of the longest product in the absence of four ribo-nucleoside triphosphate should be determined.

Concept introduction:

DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA.

RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The nucleotides are combined together by the enzyme RNA Polymerase.

Answer 6

Chapter 30, Problem 12P

Interpretation Introduction

(a)

Interpretation:

The autoradiographic pattern for strands (i) to (v) should be determined.

Concept introduction:

DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA.

RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The nucleotides are combined together by the enzyme RNA Polymerase.

Answer 7

Chapter 30, Problem 12P

Interpretation Introduction

(a)

Interpretation:

The autoradiographic pattern for strands (i) to (v) should be determined.

Concept introduction:

DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA.

RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The nucleotides are combined together by the enzyme RNA Polymerase.

Answer 8

Interpretation Introduction

(b)

Interpretation:

The possible effect of rifampicin on the RNA synthesis in the given system should be determined.

Concept introduction:

DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA.

RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The nucleotides are combined together by the enzyme RNA Polymerase.

Answer 9

Interpretation Introduction

(b)

Interpretation:

The possible effect of rifampicin on the RNA synthesis in the given system should be determined.

Concept introduction:

DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA.

RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The nucleotides are combined together by the enzyme RNA Polymerase.

Answer 10

Interpretation Introduction

(c)

Interpretation:

The reason for the difference between the heparin blocks the elongation of RNA primer before the transcription but not after should be determined.

Concept introduction:

DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA.

RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The nucleotides are combined together by the enzyme RNA Polymerase.

Answer 11

Interpretation Introduction

(c)

Interpretation:

The reason for the difference between the heparin blocks the elongation of RNA primer before the transcription but not after should be determined.

Concept introduction:

DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA.

RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The nucleotides are combined together by the enzyme RNA Polymerase.

Answer 12

Interpretation Introduction

(d)

Interpretation:

The length of the longest product in the absence of four ribo-nucleoside triphosphate should be determined.

Concept introduction:

DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA.

RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The nucleotides are combined together by the enzyme RNA Polymerase.

Answer 13

Interpretation Introduction

(d)

Interpretation:

The length of the longest product in the absence of four ribo-nucleoside triphosphate should be determined.

Concept introduction:

DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA.

RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The nucleotides are combined together by the enzyme RNA Polymerase.

Answer 14

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Answer 15

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Answer 17

Ch. 30 - Prob. 1P Ch. 30 - Prob. 2P Ch. 30 - Prob. 3P Ch. 30 - Prob. 4P Ch. 30 - Prob. 5P Ch. 30 - Prob. 6P Ch. 30 - Prob. 7P Ch. 30 - Prob. 8P Ch. 30 - Prob. 9P Ch. 30 - Prob. 10P

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