The possible products of a PCR in which one of the primers is advertently lost from the reaction mixture. Concept introduction: The polymeric chain reaction (PCR) is a technique in which amplification of a specific DNA segment occurs quickly and accurately. In this technique, a DNA sample is separated by using restriction enzymes into single strands. Then these strands are incubated with DNA polymerase, such as Taq polymerase, two primers, and deoxynucleotides triphosphate (dNTPs). The primers incorporated with the DNA polymerase to synthesize complementary strands of the target DNA.

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Answer 1

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Definition Definition Chemical process in which one or more monomers combine to produce a very large chain-like molecule called a polymer. The functional groups present on the monomers and their steric effects are responsible for polymerization through a sequence of reactions that vary in complexity. There exists a stable covalent chemical bond between monomers that sets apart polymerization from other processes.

Chapter 3, Problem 35CQ

(a)

Summary Introduction

To describe: The possible products of a PCR in which one of the primers is advertently lost from the reaction mixture.

Concept introduction:

The polymeric chain reaction (PCR) is a technique in which amplification of a specific DNA segment occurs quickly and accurately. In this technique, a DNA sample is separated by using restriction enzymes into single strands. Then these strands are incubated with DNA polymerase, such as Taq polymerase, two primers, and deoxynucleotides triphosphate (dNTPs). The primers incorporated with the DNA polymerase to synthesize complementary strands of the target DNA.

(b)

Summary Introduction

To describe: The possible products of a PCR experiment in which one of the primers is complementary to several sites in the starting DNA sample.

Concept introduction:

The polymeric chain reaction (PCR) is a technique in which amplification of a specific DNA segment occurs quickly and accurately. In this technique, a DNA sample is separated by using restriction enzymes into single strands. Then these strands are incubated with DNA polymerase such as Taq polymerase, two primers, and deoxynucleotides triphosphate (dNTPs). The primers incorporated with the DNA polymerase to synthesize complementary strands of the target DNA.

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Answer 2

Question

Definition Definition Chemical process in which one or more monomers combine to produce a very large chain-like molecule called a polymer. The functional groups present on the monomers and their steric effects are responsible for polymerization through a sequence of reactions that vary in complexity. There exists a stable covalent chemical bond between monomers that sets apart polymerization from other processes.

Chapter 3, Problem 35CQ

(a)

Summary Introduction

To describe: The possible products of a PCR in which one of the primers is advertently lost from the reaction mixture.

Concept introduction:

The polymeric chain reaction (PCR) is a technique in which amplification of a specific DNA segment occurs quickly and accurately. In this technique, a DNA sample is separated by using restriction enzymes into single strands. Then these strands are incubated with DNA polymerase, such as Taq polymerase, two primers, and deoxynucleotides triphosphate (dNTPs). The primers incorporated with the DNA polymerase to synthesize complementary strands of the target DNA.

(b)

Summary Introduction

To describe: The possible products of a PCR experiment in which one of the primers is complementary to several sites in the starting DNA sample.

Concept introduction:

The polymeric chain reaction (PCR) is a technique in which amplification of a specific DNA segment occurs quickly and accurately. In this technique, a DNA sample is separated by using restriction enzymes into single strands. Then these strands are incubated with DNA polymerase such as Taq polymerase, two primers, and deoxynucleotides triphosphate (dNTPs). The primers incorporated with the DNA polymerase to synthesize complementary strands of the target DNA.

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Answer 3

Question

Definition Definition Chemical process in which one or more monomers combine to produce a very large chain-like molecule called a polymer. The functional groups present on the monomers and their steric effects are responsible for polymerization through a sequence of reactions that vary in complexity. There exists a stable covalent chemical bond between monomers that sets apart polymerization from other processes.

Chapter 3, Problem 35CQ

(a)

Summary Introduction

To describe: The possible products of a PCR in which one of the primers is advertently lost from the reaction mixture.

Concept introduction:

The polymeric chain reaction (PCR) is a technique in which amplification of a specific DNA segment occurs quickly and accurately. In this technique, a DNA sample is separated by using restriction enzymes into single strands. Then these strands are incubated with DNA polymerase, such as Taq polymerase, two primers, and deoxynucleotides triphosphate (dNTPs). The primers incorporated with the DNA polymerase to synthesize complementary strands of the target DNA.

(b)

Summary Introduction

To describe: The possible products of a PCR experiment in which one of the primers is complementary to several sites in the starting DNA sample.

Concept introduction:

The polymeric chain reaction (PCR) is a technique in which amplification of a specific DNA segment occurs quickly and accurately. In this technique, a DNA sample is separated by using restriction enzymes into single strands. Then these strands are incubated with DNA polymerase such as Taq polymerase, two primers, and deoxynucleotides triphosphate (dNTPs). The primers incorporated with the DNA polymerase to synthesize complementary strands of the target DNA.

Expert Solution & Answer

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Answer 4

Question

Definition Definition Chemical process in which one or more monomers combine to produce a very large chain-like molecule called a polymer. The functional groups present on the monomers and their steric effects are responsible for polymerization through a sequence of reactions that vary in complexity. There exists a stable covalent chemical bond between monomers that sets apart polymerization from other processes.

Chapter 3, Problem 35CQ

(a)

Summary Introduction