FUNDAMENTALS OF BIOCHEM.-NEXTGEN ACCESS
5th Edition
ISBN: 9781119661450
Author: Voet
Publisher: WILEY
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Chapter 3, Problem 33CQ
Summary Introduction
To calculate: The number of clones necessary to obtain a probability of 0.99 and a specific 5-kb fragment of DNA from C.elegans.
Concept introduction:
Cloning is defined as the production of multiple identical organisms obtained from a single ancestor. Clone is the collection of cells that carry the vector carrying DNA of interest. To clone a particular DNA fragment, a pure form of DNA is obtained and cut using a restriction enzyme into small pieces. Then, these fragments are cloned into vector.
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HO
Fill in the missing boxes.
ON
800
NO
NO
Glucose
ATP
NADH
Hexokinase
1,3-Bisphosphoglycerate
Mg2+
ADP
NAD+, Pi
Phosphoglucose
Isomerase
Glucose-6-Phosphate
ON
沁
Glyceraldehyde-3-Phosphate
HO
حلمة
ADP
ADP Phospho
Mg2+
glycerate
Dihydroxyacetone Phosphate
ATP
kinase
ATP
Phosphoglycerate
3-phosphoglycerate
Mutase
H₁₂O
Fructose-6-Phosphate
ATP
Mg2+
ADP
Fructose-1,6-Bisphosphate
2-phosphoglycerate
H₂O
Phosphoenolpyruvate
ADP
Mg2+
ATP
Pyruvate
In a diffraction experiment of a native crystal, intensity of reflection (-1 0 6) is equivalent to the intensity of reflection (1 0 -6).
true or false?
Chapter 3 Solutions
FUNDAMENTALS OF BIOCHEM.-NEXTGEN ACCESS
Ch. 3 - Prob. 1ECh. 3 - 2. Name the following nucleotide.
Ch. 3 - 3. In many organisms, DNA is modified by...Ch. 3 - 4. When cytosine is treated with bisulfite, the...Ch. 3 - 5. Kinases are enzymes that transfer a phosphoryl...Ch. 3 - 6. Kinases are enzymes that transfer a phosphoryl...Ch. 3 - 7. A diploid organism with a 45,000-kb haploid...Ch. 3 - 8. A segment of DNA containing 20 base pairs...Ch. 3 - 9. Explain why the strands of a DNA molecule can...Ch. 3 - 10. Explain why increasing the NaCl concentration...
Ch. 3 - 11. An enzyme from the human immunodeficiency...Ch. 3 - 12. How many different amino acids could...Ch. 3 - 13. The human genome contains thousands of...Ch. 3 - 14. By how many nucleotides, on average, do the...Ch. 3 - 15. The recognition sequence for the restriction...Ch. 3 - 16. Using the data in Table 3-2, identify...Ch. 3 - 17. The 13-Mb genome of the green alga...Ch. 3 - 18. Describe how to select recombinant clones if a...Ch. 3 - Prob. 19ECh. 3 - Prob. 20ECh. 3 - 21. Why is a genomic library larger than a cDNA...Ch. 3 - 22. Why do cDNA libraries derived from different...Ch. 3 - 23. Some RNA molecules me covalently modified by...Ch. 3 - 24. Would the modified nucleoside described in...Ch. 3 - 25. Draw the tautomeric form of adenine.
Ch. 3 - 26. Draw the tautomeric form of cytosine.
Ch. 3 - 27. The pK value for N1 of adenine is 3.64,...Ch. 3 - Prob. 28CQCh. 3 - Prob. 29CQCh. 3 - 30. Hypoxanthine can also base-pair with cytosine....Ch. 3 - 31. Describe the outcome of a chain-terminator...Ch. 3 - 32. Describe the outcome of a chain-terminator...Ch. 3 - 33. Calculate the number of clones required to...Ch. 3 - 34. You are attempting to clone a 250-kb segment...Ch. 3 - 35. Describe the possible outcome of a PCR...Ch. 3 - 36. Describe the possible outcome of a PCR...Ch. 3 - 37. Write the sequences of the two 12-residue...Ch. 3 - 38. A blood slain from a crime scene and blood...Ch. 3 - Prob. 1MTE
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