The slowest migrating polypeptide among the three given polypeptides in an ion-exchange chromatography having resin beads coated with positively charged groups. Introduction: In ion-exchange chromatography, polar molecules or ions are separated on the basis of their affinity to ion exchanger. Proteins, amino acids, and small nucleotides can be separated with the help of ion exchange chromatography. Ion exchange chromatography is of two types; anion-exchange chromatography and cation-exchange chromatography. Cation-exchange chromatography is used for the separation of positively charged molecules.

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Answer 1

Question

Definition Definition Set of metabolic processes and reactions that produce energy from organic substances such as glucose. Cellular respiration takes place in the organism’s cells, where the chemical energy from nutrients or molecules of oxygen is converted into adenosine triphosphate (ATP). Cellular respiration that occurs in the presence of oxygen is called aerobic respiration; in the absence of oxygen, it is called anaerobic respiration.

Chapter 3, Problem 22P

(a)

Summary Introduction

To determine: The slowest migrating polypeptide among the three given polypeptides in an ion-exchange chromatography having resin beads coated with positively charged groups.

Introduction:

In ion-exchange chromatography, polar molecules or ions are separated on the basis of their affinity to ion exchanger. Proteins, amino acids, and small nucleotides can be separated with the help of ion exchange chromatography. Ion exchange chromatography is of two types; anion-exchange chromatography and cation-exchange chromatography. Cation-exchange chromatography is used for the separation of positively charged molecules.

(b)

Summary Introduction

To determine: The slowest migrating polypeptide among the three given polypeptides in an ion-exchange chromatography having resin beads coated with negatively charged groups.

Introduction:

In ion-exchange chromatography, polar molecules or ions are separated on the basis of their affinity to ion exchanger. Proteins, amino acids, and small nucleotides can be seprated with the help of ion exchange chromatography. Ion exchange chromatography is of two types; cation-exchange chromatography and anion-exchange chromatography. Cation-exchange chromatography is used for the separation of positively charged molecules.

(c)

Summary Introduction

To determine: The slowest migrating polypeptide among the three given polypeptides in a size-exclusion chromatography column designed to separate small peptides.

Introduction:

Size-exclusion chromatography is also termed as molecular sieve chromatography. In this procedure, the molecules are separated according to their size and molecular weight. Size-exclusion chromatography is basically used for macromolecules such as proteins.

(d)

Summary Introduction

To determine: The peptide that contains ATP-binding motif shown in the given sequence logo.

Introduction:

ATP (adenosine triphosphate) is a complex organic compound that cell produces during the process of cellular respiration. Cellular respiration is the process by which energy is produced in a cell. An ATP molecule is comprised of three basic parts; adenine, ribose sugar, and three molecules of phosphate.

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The beta-lactamase hydrolyzes the lactam-ring in penicillin. Describe the mechanism of hydrolysis, insuring to include the involvement of S, D, & K in the reaction sequence. Please help

To map the active site of beta-lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine. Why doesn't D in this hexapeptide not participate in the hydrolysis of the beta-lactam ring even though S, K, and D are involved in the catalyst?

To map the active site of -lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine. Using the experimental results described above derive the primary sequence of the active site hexapeptide. Please help!

Answer 2

Question

Definition Definition Set of metabolic processes and reactions that produce energy from organic substances such as glucose. Cellular respiration takes place in the organism’s cells, where the chemical energy from nutrients or molecules of oxygen is converted into adenosine triphosphate (ATP). Cellular respiration that occurs in the presence of oxygen is called aerobic respiration; in the absence of oxygen, it is called anaerobic respiration.

Chapter 3, Problem 22P

(a)

Summary Introduction

To determine: The slowest migrating polypeptide among the three given polypeptides in an ion-exchange chromatography having resin beads coated with positively charged groups.

Introduction:

In ion-exchange chromatography, polar molecules or ions are separated on the basis of their affinity to ion exchanger. Proteins, amino acids, and small nucleotides can be separated with the help of ion exchange chromatography. Ion exchange chromatography is of two types; anion-exchange chromatography and cation-exchange chromatography. Cation-exchange chromatography is used for the separation of positively charged molecules.

(b)

Summary Introduction

To determine: The slowest migrating polypeptide among the three given polypeptides in an ion-exchange chromatography having resin beads coated with negatively charged groups.

Introduction:

In ion-exchange chromatography, polar molecules or ions are separated on the basis of their affinity to ion exchanger. Proteins, amino acids, and small nucleotides can be seprated with the help of ion exchange chromatography. Ion exchange chromatography is of two types; cation-exchange chromatography and anion-exchange chromatography. Cation-exchange chromatography is used for the separation of positively charged molecules.

(c)

Summary Introduction

To determine: The slowest migrating polypeptide among the three given polypeptides in a size-exclusion chromatography column designed to separate small peptides.

Introduction:

Size-exclusion chromatography is also termed as molecular sieve chromatography. In this procedure, the molecules are separated according to their size and molecular weight. Size-exclusion chromatography is basically used for macromolecules such as proteins.

(d)

Summary Introduction

To determine: The peptide that contains ATP-binding motif shown in the given sequence logo.

Introduction:

ATP (adenosine triphosphate) is a complex organic compound that cell produces during the process of cellular respiration. Cellular respiration is the process by which energy is produced in a cell. An ATP molecule is comprised of three basic parts; adenine, ribose sugar, and three molecules of phosphate.

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Answer 3

Question

Definition Definition Set of metabolic processes and reactions that produce energy from organic substances such as glucose. Cellular respiration takes place in the organism’s cells, where the chemical energy from nutrients or molecules of oxygen is converted into adenosine triphosphate (ATP). Cellular respiration that occurs in the presence of oxygen is called aerobic respiration; in the absence of oxygen, it is called anaerobic respiration.

Chapter 3, Problem 22P

(a)

Summary Introduction

To determine: The slowest migrating polypeptide among the three given polypeptides in an ion-exchange chromatography having resin beads coated with positively charged groups.

Introduction:

In ion-exchange chromatography, polar molecules or ions are separated on the basis of their affinity to ion exchanger. Proteins, amino acids, and small nucleotides can be separated with the help of ion exchange chromatography. Ion exchange chromatography is of two types; anion-exchange chromatography and cation-exchange chromatography. Cation-exchange chromatography is used for the separation of positively charged molecules.

(b)

Summary Introduction

To determine: The slowest migrating polypeptide among the three given polypeptides in an ion-exchange chromatography having resin beads coated with negatively charged groups.

Introduction:

In ion-exchange chromatography, polar molecules or ions are separated on the basis of their affinity to ion exchanger. Proteins, amino acids, and small nucleotides can be seprated with the help of ion exchange chromatography. Ion exchange chromatography is of two types; cation-exchange chromatography and anion-exchange chromatography. Cation-exchange chromatography is used for the separation of positively charged molecules.

(c)

Summary Introduction

To determine: The slowest migrating polypeptide among the three given polypeptides in a size-exclusion chromatography column designed to separate small peptides.

Introduction:

Size-exclusion chromatography is also termed as molecular sieve chromatography. In this procedure, the molecules are separated according to their size and molecular weight. Size-exclusion chromatography is basically used for macromolecules such as proteins.

(d)

Summary Introduction

To determine: The peptide that contains ATP-binding motif shown in the given sequence logo.

Introduction:

ATP (adenosine triphosphate) is a complex organic compound that cell produces during the process of cellular respiration. Cellular respiration is the process by which energy is produced in a cell. An ATP molecule is comprised of three basic parts; adenine, ribose sugar, and three molecules of phosphate.

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Answer 4

Question

Definition Definition Set of metabolic processes and reactions that produce energy from organic substances such as glucose. Cellular respiration takes place in the organism’s cells, where the chemical energy from nutrients or molecules of oxygen is converted into adenosine triphosphate (ATP). Cellular respiration that occurs in the presence of oxygen is called aerobic respiration; in the absence of oxygen, it is called anaerobic respiration.

Chapter 3, Problem 22P

(a)

Summary Introduction

To determine: The slowest migrating polypeptide among the three given polypeptides in an ion-exchange chromatography having resin beads coated with positively charged groups.

Introduction:

In ion-exchange chromatography, polar molecules or ions are separated on the basis of their affinity to ion exchanger. Proteins, amino acids, and small nucleotides can be separated with the help of ion exchange chromatography. Ion exchange chromatography is of two types; anion-exchange chromatography and cation-exchange chromatography. Cation-exchange chromatography is used for the separation of positively charged molecules.

(b)

Summary Introduction

To determine: The slowest migrating polypeptide among the three given polypeptides in an ion-exchange chromatography having resin beads coated with negatively charged groups.

Introduction:

In ion-exchange chromatography, polar molecules or ions are separated on the basis of their affinity to ion exchanger. Proteins, amino acids, and small nucleotides can be seprated with the help of ion exchange chromatography. Ion exchange chromatography is of two types; cation-exchange chromatography and anion-exchange chromatography. Cation-exchange chromatography is used for the separation of positively charged molecules.

(c)

Summary Introduction

To determine: The slowest migrating polypeptide among the three given polypeptides in a size-exclusion chromatography column designed to separate small peptides.

Introduction:

Size-exclusion chromatography is also termed as molecular sieve chromatography. In this procedure, the molecules are separated according to their size and molecular weight. Size-exclusion chromatography is basically used for macromolecules such as proteins.

(d)

Summary Introduction

To determine: The peptide that contains ATP-binding motif shown in the given sequence logo.

Introduction:

ATP (adenosine triphosphate) is a complex organic compound that cell produces during the process of cellular respiration. Cellular respiration is the process by which energy is produced in a cell. An ATP molecule is comprised of three basic parts; adenine, ribose sugar, and three molecules of phosphate.

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Answer 5

Chapter 3, Problem 22P

(a)

Summary Introduction

To determine: The slowest migrating polypeptide among the three given polypeptides in an ion-exchange chromatography having resin beads coated with positively charged groups.

Introduction:

In ion-exchange chromatography, polar molecules or ions are separated on the basis of their affinity to ion exchanger. Proteins, amino acids, and small nucleotides can be separated with the help of ion exchange chromatography. Ion exchange chromatography is of two types; anion-exchange chromatography and cation-exchange chromatography. Cation-exchange chromatography is used for the separation of positively charged molecules.

(b)

Summary Introduction

To determine: The slowest migrating polypeptide among the three given polypeptides in an ion-exchange chromatography having resin beads coated with negatively charged groups.

Introduction:

In ion-exchange chromatography, polar molecules or ions are separated on the basis of their affinity to ion exchanger. Proteins, amino acids, and small nucleotides can be seprated with the help of ion exchange chromatography. Ion exchange chromatography is of two types; cation-exchange chromatography and anion-exchange chromatography. Cation-exchange chromatography is used for the separation of positively charged molecules.

(c)

Summary Introduction

To determine: The slowest migrating polypeptide among the three given polypeptides in a size-exclusion chromatography column designed to separate small peptides.

Introduction:

Size-exclusion chromatography is also termed as molecular sieve chromatography. In this procedure, the molecules are separated according to their size and molecular weight. Size-exclusion chromatography is basically used for macromolecules such as proteins.

(d)

Summary Introduction

To determine: The peptide that contains ATP-binding motif shown in the given sequence logo.

Introduction:

ATP (adenosine triphosphate) is a complex organic compound that cell produces during the process of cellular respiration. Cellular respiration is the process by which energy is produced in a cell. An ATP molecule is comprised of three basic parts; adenine, ribose sugar, and three molecules of phosphate.

Answer 6

Chapter 3, Problem 22P

(a)

Summary Introduction

To determine: The slowest migrating polypeptide among the three given polypeptides in an ion-exchange chromatography having resin beads coated with positively charged groups.

Introduction:

In ion-exchange chromatography, polar molecules or ions are separated on the basis of their affinity to ion exchanger. Proteins, amino acids, and small nucleotides can be separated with the help of ion exchange chromatography. Ion exchange chromatography is of two types; anion-exchange chromatography and cation-exchange chromatography. Cation-exchange chromatography is used for the separation of positively charged molecules.

Answer 7

Chapter 3, Problem 22P

(a)

Summary Introduction

To determine: The slowest migrating polypeptide among the three given polypeptides in an ion-exchange chromatography having resin beads coated with positively charged groups.

Introduction:

In ion-exchange chromatography, polar molecules or ions are separated on the basis of their affinity to ion exchanger. Proteins, amino acids, and small nucleotides can be separated with the help of ion exchange chromatography. Ion exchange chromatography is of two types; anion-exchange chromatography and cation-exchange chromatography. Cation-exchange chromatography is used for the separation of positively charged molecules.

Answer 8

(b)

Summary Introduction

To determine: The slowest migrating polypeptide among the three given polypeptides in an ion-exchange chromatography having resin beads coated with negatively charged groups.

Introduction:

In ion-exchange chromatography, polar molecules or ions are separated on the basis of their affinity to ion exchanger. Proteins, amino acids, and small nucleotides can be seprated with the help of ion exchange chromatography. Ion exchange chromatography is of two types; cation-exchange chromatography and anion-exchange chromatography. Cation-exchange chromatography is used for the separation of positively charged molecules.

Answer 9

(b)

Summary Introduction

To determine: The slowest migrating polypeptide among the three given polypeptides in an ion-exchange chromatography having resin beads coated with negatively charged groups.

Introduction:

In ion-exchange chromatography, polar molecules or ions are separated on the basis of their affinity to ion exchanger. Proteins, amino acids, and small nucleotides can be seprated with the help of ion exchange chromatography. Ion exchange chromatography is of two types; cation-exchange chromatography and anion-exchange chromatography. Cation-exchange chromatography is used for the separation of positively charged molecules.

Answer 10

(c)

Summary Introduction

To determine: The slowest migrating polypeptide among the three given polypeptides in a size-exclusion chromatography column designed to separate small peptides.

Introduction:

Size-exclusion chromatography is also termed as molecular sieve chromatography. In this procedure, the molecules are separated according to their size and molecular weight. Size-exclusion chromatography is basically used for macromolecules such as proteins.

Answer 11

(c)

Summary Introduction

To determine: The slowest migrating polypeptide among the three given polypeptides in a size-exclusion chromatography column designed to separate small peptides.

Introduction:

Size-exclusion chromatography is also termed as molecular sieve chromatography. In this procedure, the molecules are separated according to their size and molecular weight. Size-exclusion chromatography is basically used for macromolecules such as proteins.

Answer 12

(d)

Summary Introduction

To determine: The peptide that contains ATP-binding motif shown in the given sequence logo.

Introduction:

ATP (adenosine triphosphate) is a complex organic compound that cell produces during the process of cellular respiration. Cellular respiration is the process by which energy is produced in a cell. An ATP molecule is comprised of three basic parts; adenine, ribose sugar, and three molecules of phosphate.

Answer 13

(d)

Summary Introduction

To determine: The peptide that contains ATP-binding motif shown in the given sequence logo.

Introduction:

ATP (adenosine triphosphate) is a complex organic compound that cell produces during the process of cellular respiration. Cellular respiration is the process by which energy is produced in a cell. An ATP molecule is comprised of three basic parts; adenine, ribose sugar, and three molecules of phosphate.

Answer 14

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Answer 15

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Answer 16

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Answer 17

Ch. 3 - Prob. 1P Ch. 3 - Prob. 2P Ch. 3 - Prob. 3P Ch. 3 - Prob. 4P Ch. 3 - Prob. 5P Ch. 3 - Prob. 6P Ch. 3 - Prob. 7P Ch. 3 - Prob. 8P Ch. 3 - Prob. 9P Ch. 3 - Prob. 10P

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