
Lehninger Principles of Biochemistry 7E & SaplingPlus for Lehninger Principles of Biochemistry 7E (Six-Month Access)
7th Edition
ISBN: 9781319125882
Author: David L. Nelson, Michael M. Cox
Publisher: W. H. Freeman
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Chapter 3, Problem 8P
Summary Introduction
To determine: The approximate molecular weight of a protein containing 682 amino acids in a single polypeptide chain.
Introduction:
Amino acids are the monomer units of proteins. These are basically organic compounds consisting of a carboxylic group (
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You’ve isolated a protein and determined that the Native molecular weight of the holoenzyme is 160 kD using size exclusion chromatography. Analysis of this protein using SDS-PAGE revealed 2 bands, one at 100 kD and one at 30 kD.
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Please analze the gel electrophoresis column of the VRK1 kinase (MW: 39.71 kDa).
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Lane 1: buffer
Lane 2 : Ladder
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Lane 5: Wash
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Lane 9: Dialyzed VRK1
Lane 10: LDH
Chapter 3 Solutions
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