a) I-L-P-F Interpretation: The structure of the PTH derivative product that is obtained by Edman degradation of the peptide I-L-P-F is to be given. Concept introduction: Edmund degradation involves treatment of a peptide with phenyl isothiocyanate, followed by reaction with trifluoroacetic acid when an anilinothiazolinone (ATZ) is produced. This ATZ rearranges in the presence of acids to yield N-phenylthiohydantoin. To give: The structure of the PTH derivative product that is obtained by Edman degradation of the peptide I-L-P-F.

Question

Complete the following table. The only density needed is already given. Show your calculations in a neat and easy-to-follow manner in the space below the table. All units should be included and significant figures should be given close attention. Be sure to notice that the amount of material should be in millimoles rather than moles, and the theoretical mass of the product should in milligrams rather than grams. LOCH 3 + H2SO4 HNO 3 O=C-OCH 3 NO2 x H₂O F.W. 4.0 mL 1.3 M amount 0.50 mL in H2SO4 mg Theoretical Theoretical mmoles density 1.09

Answer 1

Question

Chapter 26.SE, Problem 45AP

Interpretation Introduction

a) I-L-P-F

Interpretation:

The structure of the PTH derivative product that is obtained by Edman degradation of the peptide I-L-P-F is to be given.

Concept introduction:

Edmund degradation involves treatment of a peptide with phenyl isothiocyanate, followed by reaction with trifluoroacetic acid when an anilinothiazolinone (ATZ) is produced. This ATZ rearranges in the presence of acids to yield N-phenylthiohydantoin.

To give:

The structure of the PTH derivative product that is obtained by Edman degradation of the peptide I-L-P-F.

Interpretation Introduction

b) D-T-S-G-A

Interpretation:

The structure of the PTH derivative product that is obtained by Edman degradation of the peptide D-T-S-G-A is to be given.

Concept introduction:

Edmund degradation involves treatment of a peptide with phenyl isothiocyanate, followed by reaction with trifluoroacetic acid when an anilinothiazolinone (ATZ) is produced. This ATZ rearranges in the presence of acids to yield N-phenylthiohydantoin.

To give:

The structure of the PTH derivative product that is obtained by Edman degradation of the peptide D-T-S-G-A.

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