Interpretation:
The separation of Cyclobarbital, thiopental, and phenobarbital by Micellar electrokinetic chromatography should be explained.
Concept introduction:
Micellar electrokinetic chromatography:
The neutral molecule are separated by Micellar electrokinetic chromatography, and they are separated by the electrophoretic mobility of the molecules.
The charge of the capillary wall is reversed, when the electro osmotic flow is towards the cathode before neutrals the anion.
From same way the reversed charge of anion before cation results the separation of each other. The more time the neutral molecule spends inside the micelle, the Longer is its migration time.
In the Micellar electrokinetic chromatography, micelles are called pseudostationary phase because they are migrate in the electrical field but their concentration ids constant and it is a part of a buffer so it is called as a pseudostationary phase.
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Quantitative Chemical Analysis 9e And Sapling Advanced Single Course For Analytical Chemistry (access Card)
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