In the elution of protein, the effect of eluent pH should be explained. Ion-Exchange Chromatography: Ion-Exchange Chromatography is separation technique, which is work in the principle of exchanging of ions based on attraction to the ion exchanger. It contains two phases, one is stationary phase and another one is mobile phase. In generally resins are act as a stationary phase, the positively charged ion exchangers attract solute anions and negatively charged ion exchangers are attract solute cations. The higher polar eluent is passed through a column the exchangers are releases the solute and they will come out from the column. In this process, the stationary phase (ion exchangers) is exchange the solute ions into eluent ions therefore it is called as Ion-exchange chromatography.

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Chapter 26, Problem 26.5P

(a)

Interpretation Introduction

Interpretation:

In the elution of protein, the effect of eluent pH should be explained.

Ion-Exchange Chromatography:

Ion-Exchange Chromatography is separation technique, which is work in the principle of exchanging of ions based on attraction to the ion exchanger.

It contains two phases, one is stationary phase and another one is mobile phase.

In generally resins are act as a stationary phase, the positively charged ion exchangers attract solute anions and negatively charged ion exchangers are attract solute cations.

The higher polar eluent is passed through a column the exchangers are releases the solute and they will come out from the column.

In this process, the stationary phase (ion exchangers) is exchange the solute ions into eluent ions therefore it is called as Ion-exchange chromatography.

(b)

Interpretation Introduction

Interpretation:

In the elution of protein, the effect gradient ionic strength should be explained.

Ion-Exchange Chromatography:

Ion-Exchange Chromatography is separation technique, which is work in the principle of exchanging of ions based on attraction to the ion exchanger.

It contains two phases, one is stationary phase and another one is mobile phase.

In generally resins are act as a stationary phase, the positively charged ion exchangers attract solute anions and negatively charged ion exchangers are attract solute cations.

The higher polar eluent is passed through a column the exchangers are releases the solute and they will come out from the column.

In this process, the stationary phase (ion exchangers) is exchange the solute ions into eluent ions therefore it is called as Ion-exchange chromatography.

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