Chapter 26, Problem 26.35P

(a)

Interpretation Introduction

Interpretation:

The required pressure difference should be calculated to inject a sample in capillary electrophoresis.

Concept introduction:

Pressure for hydrodynamic injection:

In the capillary electrophoresis, the required pressure difference is given by below equation,

$\text{ΔP}\text{=}\frac{\text{128}\text{η}{\text{L}}_{\text{t}}\text{(volume)}}{{\text{tπd}}^{\text{4}}}$

Where,

$\Delta \text{P}$ is pressure difference

$\text{d}$ is inner diameter if capillary

$\text{t}$ is injection time

$\text{η}$ is sample viscosity

$\text{L}$ is total length of the capillary

(b)

Interpretation Introduction

Interpretation:

The possibility of changing height of capillary column and desired pressure difference of given capillary electrophoresis should be explained.

Concept introduction:

Pressure for hydrodynamic injection:

In the capillary electrophoresis, the desired pressure difference difference is given by below equation,

$\text{ΔP}\text{=hg}\rho$

Where,

$\Delta \text{P}$ is pressure difference

$\text{h}$ is height of capillary tube.

$\text{ρ}$ is density of  sample

$\text{g}$ gravitational constant