Chapter 26, Problem 26.45P

Interpretation Introduction

Interpretation:

The required number of plates to separate sulfate from bromide with a resolution of 2.0 should be calculated.

Concept introduction:

Number of plates:

In the separation of chemical compounds by chromatography, the peak (signals) obtained in the given condition is known as plates, the total number of plates ain the chromatogram is given by number of plates.

$\text{N}\text{=}\frac{{\text{μ}}_{\text{app}}\text{V}}{\text{2D}}\frac{{\text{L}}_{\text{d}}}{{\text{L}}_{\text{t}}}$

Where,

${\text{L}}_{\text{d}}$ is distance to detector.

$\text{σ}$ standard deviation of Gaussian band.

${\text{L}}_{\text{t}}$ total length of column.

${\text{μ}}_{\text{app}}$ apparent mobility.